##gff-version 3
##sequence-region BBa_K190073 1 2616
BBa_K190073	.	CDS	227	1744	.	+	0	ID=BBa_K190027;Name=BBa_K190027
BBa_K190073	.	start_codon	227	229	.	+	0	ID=annotation2018217;Name=start;Parent=BBa_K190027
BBa_K190073	.	tag	1711	1741	.	+	0	ID=annotation2018221;Name=His10;Parent=BBa_K190027
BBa_K190073	.	CDS	227	1326	.	+	0	ID=annotation2018216;Name=MBP;Parent=BBa_K190027
BBa_K190073	.	stop_codon	1742	1744	.	+	0	ID=annotation2018222;Name=stop;Parent=BBa_K190027
BBa_K190073	.	CDS	1360	1710	.	+	0	ID=annotation2018220;Name=ArsR;Parent=BBa_K190027
BBa_K190073	.	sequence_feature	1327	1347	.	+	0	ID=annotation2018219;Name=TEV cleavage site;Parent=BBa_K190027
BBa_K190073	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K190073	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K190073	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K190073	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K190073	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K190073	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K190073	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K190073	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K190073	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K190073	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K190073	.	ribosome_entry_site	1753	1764	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K190073	.	conserved_region	1757	1760	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K190073	.	CDS	1771	2616	.	+	0	ID=BBa_K190028;Name=BBa_K190028
BBa_K190073	.	CDS	1771	2616	.	+	0	ID=annotation2061212;Name=GlpF;Parent=BBa_K190028
BBa_K190073	.	start_codon	1771	1773	.	+	0	ID=annotation2018038;Name=start;Parent=BBa_K190028
>BBa_K190073
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaatcga
agaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaa
gataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatg
gccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccgga
caaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatc
gctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgc
tggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgat
tgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggc
gcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcag
aagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagt
gaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtatt
aacgccgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcgg
ttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgc
cactatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtact
gcggtgatcaacgccgccagcggtcgtcagactgtcgatgcagccctggcagctgcgcagactgaagagctctacttcc
agggtgccgccgccgccatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcat
cgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatc
tcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttat
caccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgt
ccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagtcatcatcatcaccatcatcatcaccat
cattaatactagagaaagaggagaaatactagatgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttct
cggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgg
gaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatc
ccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgc
cggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacatt
gttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcagg
ctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtacc
acgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggtttt
gccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcg
gcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaa
actgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagct
tcgctgtaa