LOCUS BBa_K1903002 4400 bp DNA linear 22-Nov-2024 DEFINITION dCas9 with protein insertion frames version B ACCESSION BBa_K1903002 VERSION BBa_K1903002.1 FEATURES Location/Qualifiers misc_feature 1..4134 /label=dCas9 B version CDS 1..4263 /label=BBa_K1903017 stem_loop 4283..4326 /label=stem_loop misc_feature 4272..4351 /label=BBa_B0010 terminator 4272..4351 /label=BBa_B0010 stem_loop 4367..4386 /label=T7 TE misc_feature 4393..4393 /label=stop polyA_site 4387..4400 /label=polya misc_feature 4360..4400 /label=BBa_B0012 terminator 4360..4400 /label=BBa_B0012 terminator 4272..4400 /label=BBa_B0015 ORIGIN 1 atggacaaga aatattcgat tggtcttgcc atcgggacga actccgtagg gtgggcggtc 61 atcaccgacg agtacaaggt accgtcaaaa aagtttaaag tcttgggaaa tacggaccgc 121 cactcaatca agaaaaattt gattggggcc ctgctgtttg attccggaga gacagcagaa 181 gccacacgtt tgaagcgcac tgcacgccgc cgctacacgc gtcgtaaaaa ccgcatctgc 241 tatttgcagg aaattttctc gaacgagatg gcaaaggtcg atgacagctt ttttcatcgt 301 ctggaggaaa gttttttggt agaagaggac aaaaagcatg aacgccaccc tatcttcggg 361 aacattgtag atgaagtcgc ttaccatgag aagtatccga ctatttatca tcttcgcaaa 421 aagctggtag attctaccga taaagcggac ttgcgcttga tttacttggc gctggcgcac 481 atgatcaagt tccgtggcca cttcttaatt gaaggtgacc tgaaccccga caattcagat 541 gtggacaagc tgttcattca actggtacaa acctataatc agttgttcga agaaaaccca 601 attaatgcgt ccggagtgga cgcaaaggct atcttatctg cacgcttaag caagtctcgt 661 cgtttagaga atcttattgc acagttaccg ggagagaaaa agaacggatt attcggaaat 721 cttatcgcgc ttagtcttgg attaacccct aatttcaaaa gtaacttcga cttagctgag 781 gatgcgaaat tgcagttatc caaggacact tacgatgatg accttgacaa cctgctggcg 841 cagattggtg atcagtacgc ggacttgttt ctggcggcga aaaatttgtc tgacgccatt 901 cttttgtctg atatccttcg tgttaatact gagattacca aggcgcccct ttcggcctca 961 atgattaaac gttacgacga gcaccatcag gacttgactc ttctgaaggc tcttgttcgc 1021 cagcaacttc cggaaaagta caaagagatc ttcttcgatc agtccaagaa cggctacgct 1081 gggtacattg acggaggcgc ttcgcaagaa gagttctaca agtttattaa accgattctg 1141 gagaagatgg acggtacgga agagctgtta gttaagctga atcgcgagga cctgttacgc 1201 aagcaacgca cgttcgacaa tggctccatt ccacatcaaa tccatctggg tgagttacat 1261 gcaattctgc gccgccaaga ggatttctat ccatttctta aagataaccg cgagaagatt 1321 gaaaaaatct tgacatttcg tatcccttat tatgtggggc cgttagcccg tgggaacagc 1381 cgcttcgcat ggatgacgcg caagtcggaa gagacaatca caccctggaa ttttgaggag 1441 gtagtggaca aaggcgccag cgctcaatcc tttattgaac gtatgactaa ttttgacaaa 1501 aatttgccta acgaaaaagt cctgccgaag cacagcctgt tatatgaata ctttacggtt 1561 tataacgaat taacaaaagt taagtacgtg accgagggaa tgcgcaaacc cgcattcctt 1621 tccggcgagc aaaaaaaagc gattgtcgat cttctgttca aaacaaaccg caaggttaca 1681 gtgaagcagc ttaaggaaga ttactttaag aaaattgaat gtttcgattc tgtcgaaatt 1741 tcgggtgtgg aggagtcttc tcgtttcaac gcaagtcttg ggacgtatca cgacgaagac 1801 ttgcttaaga tcatcaaaga caaggacttc cttgataatg aggagaatga ggacatcttg 1861 gaggacatcg tgttgacctt aactctgttt gaggatcgtg agatgatcga agaacgcctt 1921 aagacgtacg cacacttatt tgacgacaaa gttatgaaac agctgaagcg ccgtcgctac 1981 accgggtggg gacgtttatc acgcaagctt atcaacggaa tccgtgataa acaatcgggc 2041 aagacgatcc ttgactttct gaagtcggac ggttttgcga accgtaattt catgcaatta 2101 attcatgacg attctctgac atttaaagag gacatccaga aagcccaagt gtctggacag 2161 ggtgacagcc tgcatgaaca cattgcgaac ttagcgggct ctccggcaat taagaaggga 2221 atcttgcaaa cggtgaaggt ggtggatgag ttggtgaaag ttatgggtcg ccacaagcca 2281 gaaaatattg taatcgagat ggcacgcgag aaccaaacaa cccaaaaggg ccaaaagaac 2341 agccgtgagc gcatgaaacg tatcgaggag ggtatcaagg aattagggtc gcaaatcttg 2401 aaagaacatc cagttgagaa cacgcaactg caaaacgaga aattgtacct ttattattta 2461 caaaatgggc gcgatatgta cgttgaccaa gagttggata ttaatcgttt gtcggactac 2521 gacgtggacg caattgtgcc acagtctttc cttaaagatg attctattga taataaagtc 2581 ctgacacgtt ctgataagaa ccgtggtaag agcgacaacg tcccatctga agaagtagtg 2641 aaaaaaatga agtcttcaaa ttactggcgt cagttattaa atgcgaaatt gattacgcag 2701 cgcaagttcg acaacgaaga ccttactaaa gctgagcgtg ggggtctgtc cgaactggac 2761 aaagcagggt tcattaaacg ccaattggtg gagacacgtc aaattaccaa gcatgttgca 2821 cagatcttag actctcgtat gaataccaaa tacgacgaaa atgataagct gatccgtgaa 2881 gtcaaagtta ttactcttaa gtccaagctg gtgtccgact tccgtaaaga ttttcagttc 2941 tacaaggtac gcgaaatcaa taactatcac cacgcccatg acgcgtattt aaatgcggta 3001 gtaggtaccg ctctgatcaa aaagtatccc aaactggaga gcgagttcgt ttatggcgac 3061 tataaagttt acgatgtacg taagatgatt gcaaaatccg aacaagaaat cgggaaagcc 3121 actgcgaaat actttttcta ctcaaacatt atgaacttct ttaagacgga aattacttta 3181 gccaacggcg agattcgcaa acgcccttta attgaaacaa atggcgagac gggggagatt 3241 gtatgggaca aaggacgcga tttcgcaacc gttcgcaaag tacttagtat gccacaggtg 3301 aatatcgtaa agaaaacgga agtgcagact ggcggcttca gcaaagaatc aatccttccc 3361 aagcgcaatt cagacaagct gattgcacgc aagaaggatt gggaccctaa aaagtatggc 3421 gggtttgaca gccccaccgt agcgtattct gtgttagtcg tcgcaaaagt ggaaaagggt 3481 aaatcgaaga agcttaaatc tgtgaaagaa cttttgggaa tcacaattat ggaacgctct 3541 tctttcgaga aaaaccctat tgacttcttg gaagctaagg ggtacaagga ggtaaaaaag 3601 gatttgatca ttaagttgcc caagtactca ttattcgagc tggaaaacgg acgcaagcgt 3661 atgctggcct ctgctggcga gttgcaaaaa ggaaacgaac ttgccctgcc gtcaaaatac 3721 gttaacttcc tgtatttggc ttctcattac gaaaaactga agggtagccc tgaagataat 3781 gagcagaaac aactttttgt tgagcaacat aaacattatc ttgatgaaat tattgagcag 3841 attagcgaat ttagcaaacg tgtcatctta gctgacgcta accttgacaa agtgttaagc 3901 gcctacaaca aacatcgtga taaacctatt cgtgaacaag ccgagaatat tatccacttg 3961 tttacactga cgaatttagg tgctccggcc gcttttaagt atttcgatac caccattgat 4021 cgcaagcgct atacttctac gaaggaggtg cttgatgcaa ctcttattca ccaatcaatc 4081 acgggtctgt atgaaacgcg cattgatttg agtcagttgg gcggtgatta ataaccaggc 4141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 4201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 4261 atatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 4321 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 4381 tgggcctttc tgcgtttata //