BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1903002 1 dCas9 B dCas9 with protein insertion frames version B 2016-10-13T11:00:00Z 2016-10-17T07:04:20Z Yes dCas9 false false _2369_ 29474 29450 9 true Golden Gate false Sof??a Vieto Fonseca component2516032 1 BBa_K1903017 component2516039 1 BBa_B0015 annotation2516032 1 BBa_K1903017 range2516032 1 1 4263 annotation2516039 1 BBa_B0015 range2516039 1 4272 4400 BBa_K1903017 1 BBa_K1903017 dCas9 B coding sequence 2016-10-16T11:00:00Z 2016-10-17T07:05:41Z The source for the Cas9 sequence was UNIPROT accession: Q99ZW2. We optimized the sequence for E. coli and changed the amino acids at positions D10A and H840A for making the protein catalytically inactive. The sequence is a coding part for a dCAs9 protein assembly with golden gate and has two windows frames for protein insertions at amino acids: N588 and N888 false false _2369_ 29474 29474 9 false This CDS was assembled with Golden Gate using four gBlocks. false Rafael Montenegro Mar??n annotation2516413 1 dCas9 B version range2516413 1 1 4134 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1903017_sequence 1 atggacaagaaatattcgattggtcttgccatcgggacgaactccgtagggtgggcggtcatcaccgacgagtacaaggtaccgtcaaaaaagtttaaagtcttgggaaatacggaccgccactcaatcaagaaaaatttgattggggccctgctgtttgattccggagagacagcagaagccacacgtttgaagcgcactgcacgccgccgctacacgcgtcgtaaaaaccgcatctgctatttgcaggaaattttctcgaacgagatggcaaaggtcgatgacagcttttttcatcgtctggaggaaagttttttggtagaagaggacaaaaagcatgaacgccaccctatcttcgggaacattgtagatgaagtcgcttaccatgagaagtatccgactatttatcatcttcgcaaaaagctggtagattctaccgataaagcggacttgcgcttgatttacttggcgctggcgcacatgatcaagttccgtggccacttcttaattgaaggtgacctgaaccccgacaattcagatgtggacaagctgttcattcaactggtacaaacctataatcagttgttcgaagaaaacccaattaatgcgtccggagtggacgcaaaggctatcttatctgcacgcttaagcaagtctcgtcgtttagagaatcttattgcacagttaccgggagagaaaaagaacggattattcggaaatcttatcgcgcttagtcttggattaacccctaatttcaaaagtaacttcgacttagctgaggatgcgaaattgcagttatccaaggacacttacgatgatgaccttgacaacctgctggcgcagattggtgatcagtacgcggacttgtttctggcggcgaaaaatttgtctgacgccattcttttgtctgatatccttcgtgttaatactgagattaccaaggcgcccctttcggcctcaatgattaaacgttacgacgagcaccatcaggacttgactcttctgaaggctcttgttcgccagcaacttccggaaaagtacaaagagatcttcttcgatcagtccaagaacggctacgctgggtacattgacggaggcgcttcgcaagaagagttctacaagtttattaaaccgattctggagaagatggacggtacggaagagctgttagttaagctgaatcgcgaggacctgttacgcaagcaacgcacgttcgacaatggctccattccacatcaaatccatctgggtgagttacatgcaattctgcgccgccaagaggatttctatccatttcttaaagataaccgcgagaagattgaaaaaatcttgacatttcgtatcccttattatgtggggccgttagcccgtgggaacagccgcttcgcatggatgacgcgcaagtcggaagagacaatcacaccctggaattttgaggaggtagtggacaaaggcgccagcgctcaatcctttattgaacgtatgactaattttgacaaaaatttgcctaacgaaaaagtcctgccgaagcacagcctgttatatgaatactttacggtttataacgaattaacaaaagttaagtacgtgaccgagggaatgcgcaaacccgcattcctttccggcgagcaaaaaaaagcgattgtcgatcttctgttcaaaacaaaccgcaaggttacagtgaagcagcttaaggaagattactttaagaaaattgaatgtttcgattctgtcgaaatttcgggtgtggaggagtcttctcgtttcaacgcaagtcttgggacgtatcacgacgaagacttgcttaagatcatcaaagacaaggacttccttgataatgaggagaatgaggacatcttggaggacatcgtgttgaccttaactctgtttgaggatcgtgagatgatcgaagaacgccttaagacgtacgcacacttatttgacgacaaagttatgaaacagctgaagcgccgtcgctacaccgggtggggacgtttatcacgcaagcttatcaacggaatccgtgataaacaatcgggcaagacgatccttgactttctgaagtcggacggttttgcgaaccgtaatttcatgcaattaattcatgacgattctctgacatttaaagaggacatccagaaagcccaagtgtctggacagggtgacagcctgcatgaacacattgcgaacttagcgggctctccggcaattaagaagggaatcttgcaaacggtgaaggtggtggatgagttggtgaaagttatgggtcgccacaagccagaaaatattgtaatcgagatggcacgcgagaaccaaacaacccaaaagggccaaaagaacagccgtgagcgcatgaaacgtatcgaggagggtatcaaggaattagggtcgcaaatcttgaaagaacatccagttgagaacacgcaactgcaaaacgagaaattgtacctttattatttacaaaatgggcgcgatatgtacgttgaccaagagttggatattaatcgtttgtcggactacgacgtggacgcaattgtgccacagtctttccttaaagatgattctattgataataaagtcctgacacgttctgataagaaccgtggtaagagcgacaacgtcccatctgaagaagtagtgaaaaaaatgaagtcttcaaattactggcgtcagttattaaatgcgaaattgattacgcagcgcaagttcgacaacgaagaccttactaaagctgagcgtgggggtctgtccgaactggacaaagcagggttcattaaacgccaattggtggagacacgtcaaattaccaagcatgttgcacagatcttagactctcgtatgaataccaaatacgacgaaaatgataagctgatccgtgaagtcaaagttattactcttaagtccaagctggtgtccgacttccgtaaagattttcagttctacaaggtacgcgaaatcaataactatcaccacgcccatgacgcgtatttaaatgcggtagtaggtaccgctctgatcaaaaagtatcccaaactggagagcgagttcgtttatggcgactataaagtttacgatgtacgtaagatgattgcaaaatccgaacaagaaatcgggaaagccactgcgaaatactttttctactcaaacattatgaacttctttaagacggaaattactttagccaacggcgagattcgcaaacgccctttaattgaaacaaatggcgagacgggggagattgtatgggacaaaggacgcgatttcgcaaccgttcgcaaagtacttagtatgccacaggtgaatatcgtaaagaaaacggaagtgcagactggcggcttcagcaaagaatcaatccttcccaagcgcaattcagacaagctgattgcacgcaagaaggattgggaccctaaaaagtatggcgggtttgacagccccaccgtagcgtattctgtgttagtcgtcgcaaaagtggaaaagggtaaatcgaagaagcttaaatctgtgaaagaacttttgggaatcacaattatggaacgctcttctttcgagaaaaaccctattgacttcttggaagctaaggggtacaaggaggtaaaaaaggatttgatcattaagttgcccaagtactcattattcgagctggaaaacggacgcaagcgtatgctggcctctgctggcgagttgcaaaaaggaaacgaacttgccctgccgtcaaaatacgttaacttcctgtatttggcttctcattacgaaaaactgaagggtagccctgaagataatgagcagaaacaactttttgttgagcaacataaacattatcttgatgaaattattgagcagattagcgaatttagcaaacgtgtcatcttagctgacgctaaccttgacaaagtgttaagcgcctacaacaaacatcgtgataaacctattcgtgaacaagccgagaatattatccacttgtttacactgacgaatttaggtgctccggccgcttttaagtatttcgataccaccattgatcgcaagcgctatacttctacgaaggaggtgcttgatgcaactcttattcaccaatcaatcacgggtctgtatgaaacgcgcattgatttgagtcagttgggcggtgattaataa BBa_K1903002_sequence 1 atggacaagaaatattcgattggtcttgccatcgggacgaactccgtagggtgggcggtcatcaccgacgagtacaaggtaccgtcaaaaaagtttaaagtcttgggaaatacggaccgccactcaatcaagaaaaatttgattggggccctgctgtttgattccggagagacagcagaagccacacgtttgaagcgcactgcacgccgccgctacacgcgtcgtaaaaaccgcatctgctatttgcaggaaattttctcgaacgagatggcaaaggtcgatgacagcttttttcatcgtctggaggaaagttttttggtagaagaggacaaaaagcatgaacgccaccctatcttcgggaacattgtagatgaagtcgcttaccatgagaagtatccgactatttatcatcttcgcaaaaagctggtagattctaccgataaagcggacttgcgcttgatttacttggcgctggcgcacatgatcaagttccgtggccacttcttaattgaaggtgacctgaaccccgacaattcagatgtggacaagctgttcattcaactggtacaaacctataatcagttgttcgaagaaaacccaattaatgcgtccggagtggacgcaaaggctatcttatctgcacgcttaagcaagtctcgtcgtttagagaatcttattgcacagttaccgggagagaaaaagaacggattattcggaaatcttatcgcgcttagtcttggattaacccctaatttcaaaagtaacttcgacttagctgaggatgcgaaattgcagttatccaaggacacttacgatgatgaccttgacaacctgctggcgcagattggtgatcagtacgcggacttgtttctggcggcgaaaaatttgtctgacgccattcttttgtctgatatccttcgtgttaatactgagattaccaaggcgcccctttcggcctcaatgattaaacgttacgacgagcaccatcaggacttgactcttctgaaggctcttgttcgccagcaacttccggaaaagtacaaagagatcttcttcgatcagtccaagaacggctacgctgggtacattgacggaggcgcttcgcaagaagagttctacaagtttattaaaccgattctggagaagatggacggtacggaagagctgttagttaagctgaatcgcgaggacctgttacgcaagcaacgcacgttcgacaatggctccattccacatcaaatccatctgggtgagttacatgcaattctgcgccgccaagaggatttctatccatttcttaaagataaccgcgagaagattgaaaaaatcttgacatttcgtatcccttattatgtggggccgttagcccgtgggaacagccgcttcgcatggatgacgcgcaagtcggaagagacaatcacaccctggaattttgaggaggtagtggacaaaggcgccagcgctcaatcctttattgaacgtatgactaattttgacaaaaatttgcctaacgaaaaagtcctgccgaagcacagcctgttatatgaatactttacggtttataacgaattaacaaaagttaagtacgtgaccgagggaatgcgcaaacccgcattcctttccggcgagcaaaaaaaagcgattgtcgatcttctgttcaaaacaaaccgcaaggttacagtgaagcagcttaaggaagattactttaagaaaattgaatgtttcgattctgtcgaaatttcgggtgtggaggagtcttctcgtttcaacgcaagtcttgggacgtatcacgacgaagacttgcttaagatcatcaaagacaaggacttccttgataatgaggagaatgaggacatcttggaggacatcgtgttgaccttaactctgtttgaggatcgtgagatgatcgaagaacgccttaagacgtacgcacacttatttgacgacaaagttatgaaacagctgaagcgccgtcgctacaccgggtggggacgtttatcacgcaagcttatcaacggaatccgtgataaacaatcgggcaagacgatccttgactttctgaagtcggacggttttgcgaaccgtaatttcatgcaattaattcatgacgattctctgacatttaaagaggacatccagaaagcccaagtgtctggacagggtgacagcctgcatgaacacattgcgaacttagcgggctctccggcaattaagaagggaatcttgcaaacggtgaaggtggtggatgagttggtgaaagttatgggtcgccacaagccagaaaatattgtaatcgagatggcacgcgagaaccaaacaacccaaaagggccaaaagaacagccgtgagcgcatgaaacgtatcgaggagggtatcaaggaattagggtcgcaaatcttgaaagaacatccagttgagaacacgcaactgcaaaacgagaaattgtacctttattatttacaaaatgggcgcgatatgtacgttgaccaagagttggatattaatcgtttgtcggactacgacgtggacgcaattgtgccacagtctttccttaaagatgattctattgataataaagtcctgacacgttctgataagaaccgtggtaagagcgacaacgtcccatctgaagaagtagtgaaaaaaatgaagtcttcaaattactggcgtcagttattaaatgcgaaattgattacgcagcgcaagttcgacaacgaagaccttactaaagctgagcgtgggggtctgtccgaactggacaaagcagggttcattaaacgccaattggtggagacacgtcaaattaccaagcatgttgcacagatcttagactctcgtatgaataccaaatacgacgaaaatgataagctgatccgtgaagtcaaagttattactcttaagtccaagctggtgtccgacttccgtaaagattttcagttctacaaggtacgcgaaatcaataactatcaccacgcccatgacgcgtatttaaatgcggtagtaggtaccgctctgatcaaaaagtatcccaaactggagagcgagttcgtttatggcgactataaagtttacgatgtacgtaagatgattgcaaaatccgaacaagaaatcgggaaagccactgcgaaatactttttctactcaaacattatgaacttctttaagacggaaattactttagccaacggcgagattcgcaaacgccctttaattgaaacaaatggcgagacgggggagattgtatgggacaaaggacgcgatttcgcaaccgttcgcaaagtacttagtatgccacaggtgaatatcgtaaagaaaacggaagtgcagactggcggcttcagcaaagaatcaatccttcccaagcgcaattcagacaagctgattgcacgcaagaaggattgggaccctaaaaagtatggcgggtttgacagccccaccgtagcgtattctgtgttagtcgtcgcaaaagtggaaaagggtaaatcgaagaagcttaaatctgtgaaagaacttttgggaatcacaattatggaacgctcttctttcgagaaaaaccctattgacttcttggaagctaaggggtacaaggaggtaaaaaaggatttgatcattaagttgcccaagtactcattattcgagctggaaaacggacgcaagcgtatgctggcctctgctggcgagttgcaaaaaggaaacgaacttgccctgccgtcaaaatacgttaacttcctgtatttggcttctcattacgaaaaactgaagggtagccctgaagataatgagcagaaacaactttttgttgagcaacataaacattatcttgatgaaattattgagcagattagcgaatttagcaaacgtgtcatcttagctgacgctaaccttgacaaagtgttaagcgcctacaacaaacatcgtgataaacctattcgtgaacaagccgagaatattatccacttgtttacactgacgaatttaggtgctccggccgcttttaagtatttcgataccaccattgatcgcaagcgctatacttctacgaaggaggtgcttgatgcaactcttattcaccaatcaatcacgggtctgtatgaaacgcgcattgatttgagtcagttgggcggtgattaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z