LOCUS       BBa_K1903004            1611 bp    DNA     linear       26-Nov-2024
DEFINITION  GFP-TEVlinker-Quencher
ACCESSION   BBa_K1903004
VERSION     BBa_K1903004.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP protein
     CDS             1..720
                     /label=BBa_E0040
     misc_feature    732..752
                     /label=TEV cleavage site
     CDS             729..755
                     /label=BBa_K1159318
     CDS             764..1474
                     /label=REACh1
     CDS             764..1474
                     /label=BBa_K1319001
     stem_loop       1494..1537
                     /label=stem_loop
     misc_feature    1483..1562
                     /label=BBa_B0010
     terminator      1483..1562
                     /label=BBa_B0010
     stem_loop       1578..1597
                     /label=T7 TE
     misc_feature    1604..1604
                     /label=stop
     polyA_site      1598..1611
                     /label=polya
     misc_feature    1571..1611
                     /label=BBa_B0012
     terminator      1571..1611
                     /label=BBa_B0012
     terminator      1483..1611
                     /label=BBa_B0015
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagaggg agagaatttg tattttcagt caggatacta gaggtgagca agggcgagga
      781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc atcgtgacca ccttcggcta
      961 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc
     1021 cgccatgccc gaaggctacg accaggagcg caccatcttc ttcaaggacg acggcaacta
     1081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactggaa
     1201 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1381 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1441 cgccgggatc actctcggca tggacgagct gtactactag agccaggcat caaataaaac
     1501 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1561 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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