LOCUS       BBa_K1903006            1029 bp    DNA     linear       31-Oct-2024
DEFINITION  Split TEV N-IntN DnaE Device
ACCESSION   BBa_K1903006
VERSION     BBa_K1903006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..582
                     /label=N-terminal half of TEV Protease
     CDS             229..582
                     /label=BBa_K1159101
     misc_feature    591..892
                     /label=BBa_K1362400
     stem_loop       912..955
                     /label=stem_loop
     misc_feature    901..980
                     /label=BBa_B0010
     terminator      901..980
                     /label=BBa_B0010
     stem_loop       996..1015
                     /label=T7 TE
     misc_feature    1022..1022
                     /label=stop
     polyA_site      1016..1029
                     /label=polya
     misc_feature    989..1029
                     /label=BBa_B0012
     terminator      989..1029
                     /label=BBa_B0012
     terminator      901..1029
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaggg agaaagcttg
      241 tttaaggggc cgcgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa
      301 tctgatgggc acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac
      361 aagcacttgt ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc
      421 aaggtcaaga acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt
      481 attcgcatgc ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa
      541 agggaagagc gcatatgtct tgtgacaacc aacttccaaa cttactagag taagctatga
      601 aacggaaata ttgacagtag aatatggatt attaccgatt ggtaaaattg tagaaaagcg
      661 catcgaatgt actgtttata gcgttgataa taatggaaat atttatacac aacctgtagc
      721 acaatggcac gatcgcggag aacaagaggt gtttgagtat tgtttggaag atggttcatt
      781 gattcgggca acaaaagacc ataagtttat gactgttgat ggtcaaatgt tgccaattga
      841 tgaaatattt gaacgtgaat tggatttgat gcgggttgat aatttgccga attactagag
      901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1021 gcgtttata
//