LOCUS       BBa_K1903007             600 bp    DNA     linear       31-Dec-2025
DEFINITION  IntC DnaE-Split TEV
ACCESSION   BBa_K1903007
VERSION     BBa_K1903007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..101
                     /label=BBa_K1362401
     CDS             110..463
                     /label=C-terminal half of TEV Protease
     misc_feature    410..412
                     /label=Ser --> Val for autolysis resistance
     CDS             110..463
                     /label=BBa_K1159102
     stem_loop       483..526
                     /label=stem_loop
     misc_feature    472..551
                     /label=BBa_B0010
     terminator      472..551
                     /label=BBa_B0010
     stem_loop       567..586
                     /label=T7 TE
     misc_feature    593..593
                     /label=stop
     polyA_site      587..600
                     /label=polya
     misc_feature    560..600
                     /label=BBa_B0012
     terminator      560..600
                     /label=BBa_B0012
     terminator      472..600
                     /label=BBa_B0015
ORIGIN
        1 atcaaaatag ccacacgtaa atatttaggc aaacaaaatg tctatgacat tggagttgag
       61 cgcgaccata attttgcact caaaaatggc ttcatagctt ctactagaga agagcatgtc
      121 tagcatggtg tcagacacta gctgcacatt cccttcatct gatggcatat tctggaagca
      181 ttggattcaa accaaggatg ggcagtgtgg cagtccatta gtatcaacta gagatgggtt
      241 cattgttggt atacactcag catcgaattt caccaacaca aacaattatt tcacaagcgt
      301 gccgaaaaac ttcatggaat tgttgacaaa tcaggaggcg cagcagtggg ttagtggttg
      361 gcgattaaat gctgactcag tattgtgggg gggccataaa gttttcatgg tgaaacctga
      421 agagcctttt cagccagtta aggaagcgac tcaactcatg aattactaga gccaggcatc
      481 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      541 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//