LOCUS       BBa_K1903008             663 bp    DNA     linear       22-Dec-2024
DEFINITION  Split TEV-IntC DnaE Device
ACCESSION   BBa_K1903008
VERSION     BBa_K1903008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..164
                     /label=BBa_K1362401
     CDS             173..526
                     /label=C-terminal half of TEV Protease
     misc_feature    473..475
                     /label=Ser --> Val for autolysis resistance
     CDS             173..526
                     /label=BBa_K1159102
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     terminator      535..663
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagatcaaaa tagccacacg taaatattta ggcaaacaaa atgtctatga cattggagtt
      121 gagcgcgacc ataattttgc actcaaaaat ggcttcatag cttctactag agaagagcat
      181 gtctagcatg gtgtcagaca ctagctgcac attcccttca tctgatggca tattctggaa
      241 gcattggatt caaaccaagg atgggcagtg tggcagtcca ttagtatcaa ctagagatgg
      301 gttcattgtt ggtatacact cagcatcgaa tttcaccaac acaaacaatt atttcacaag
      361 cgtgccgaaa aacttcatgg aattgttgac aaatcaggag gcgcagcagt gggttagtgg
      421 ttggcgatta aatgctgact cagtattgtg ggggggccat aaagttttca tggtgaaacc
      481 tgaagagcct tttcagccag ttaaggaagc gactcaactc atgaattact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 ata
//