LOCUS       BBa_K1903012             663 bp    DNA     linear       25-Jan-2026
DEFINITION  PA1-RBS + IntC DnaB
ACCESSION   BBa_K1903012
VERSION     BBa_K1903012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=start
     misc_feature    524..526
                     /label=stop
     misc_feature    521..523
                     /label=stop
     misc_feature    65..82
                     /label=tag
     misc_feature    83..511
                     /label=SspDnaB C intein
     misc_feature    512..520
                     /label=SspDnaB C extein
     misc_feature    509..511
                     /label=N -> A
     CDS             62..526
                     /label=BBa_K1362251
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     terminator      535..663
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcatcat caccatcacc acagcacagg aaaaagagtt tctattaaag atttgttaga
      121 tgaaaaagat tttgaaatat gggcaattaa tgaacagacg atgaagctag aatcagctaa
      181 agttagtcgt gtattttgta ctggcaaaaa gctagtttat attctaaaaa ctcgactagg
      241 tagaactatc aaggcaacag caaatcatag atttttaact attgatggtt ggaaaagatt
      301 agatgagcta tctttaaaag agcatattgc tctaccccgt aaactagaaa gctcctcttt
      361 acaattgtca ccagaaatag aaaagttgtc tcagagtgat atttactggg actccatcgt
      421 ttctattacg gagactggag tcgaagaggt ttttgatttg actgtgccag gaccacataa
      481 ctttgtcgcc aatgacatca ttgtccatgc ctcaggcggg taataatact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 ata
//