##gff-version 3
##sequence-region BBa_K1903013 1 1700
BBa_K1903013	.	CDS	1	1029	.	+	0	ID=BBa_K1903006;Name=BBa_K1903006
BBa_K1903013	.	terminator	901	1029	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1903006
BBa_K1903013	.	terminator	989	1029	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1903013	.	stem_loop	996	1015	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1903013	.	engineered_region	989	1029	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1903013	.	stop_codon	1022	1022	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1903013	.	polyA_site	1016	1029	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1903013	.	terminator	901	980	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1903013	.	engineered_region	901	980	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1903013	.	stem_loop	912	955	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1903013	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1903006
BBa_K1903013	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1903013	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1903013	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1903013	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1903013	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1903013	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1903013	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1903013	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1903006
BBa_K1903013	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1903013	.	CDS	229	582	.	+	0	ID=BBa_K1159101;Name=BBa_K1159101;Parent=BBa_K1903006
BBa_K1903013	.	CDS	229	582	.	+	0	ID=annotation2341473;Name=N-terminal half of TEV Protease;Parent=BBa_K1159101
BBa_K1903013	.	polypeptide_domain	591	892	.	+	0	ID=BBa_K1362400;Name=BBa_K1362400;Parent=BBa_K1903006
BBa_K1903013	.	CDS	1038	1700	.	+	0	ID=BBa_K1903008;Name=BBa_K1903008
BBa_K1903013	.	promoter	1038	1072	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1903008
BBa_K1903013	.	terminator	1572	1700	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1903008
BBa_K1903013	.	terminator	1660	1700	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1903013	.	stem_loop	1667	1686	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1903013	.	engineered_region	1660	1700	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1903013	.	stop_codon	1693	1693	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1903013	.	polyA_site	1687	1700	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1903013	.	terminator	1572	1651	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1903013	.	engineered_region	1572	1651	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1903013	.	stem_loop	1583	1626	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1903013	.	CDS	1210	1563	.	+	0	ID=BBa_K1159102;Name=BBa_K1159102;Parent=BBa_K1903008
BBa_K1903013	.	sequence_alteration	1510	1512	.	+	0	ID=annotation2341475;Name=Ser --> Val for autolysis resistance;Parent=BBa_K1159102
BBa_K1903013	.	CDS	1210	1563	.	+	0	ID=annotation2341474;Name=C-terminal half of TEV Protease;Parent=BBa_K1159102
BBa_K1903013	.	ribosome_entry_site	1081	1092	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1903008
BBa_K1903013	.	conserved_region	1085	1088	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1903013	.	polypeptide_domain	1101	1201	.	+	0	ID=BBa_K1362401;Name=BBa_K1362401;Parent=BBa_K1903008
>BBa_K1903013
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagggagaaagc
ttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaa
catcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgtt
ggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatg
ataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgca
tatgtcttgtgacaaccaacttccaaacttactagagtaagctatgaaacggaaatattgacagtagaatatggattat
taccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacaca
acctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggca
acaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttga
tgcgggttgataatttgccgaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagatcaaa
atagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaa
atggcttcatagcttctactagagaagagcatgtctagcatggtgtcagacactagctgcacattcccttcatctgatg
gcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcat
tgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattg
ttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaag
ttttcatggtgaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgaattactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata