LOCUS       BBa_K1903014            1796 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac-RBS-RFP-IntN DnaB + PA1-RBS-IntC DnaB
ACCESSION   BBa_K1903014
VERSION     BBa_K1903014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..932
                     /label=BBa_E1010
     misc_feature    941..988
                     /label=Int N DnaB 
     CDS             941..988
                     /label=BBa_K1903019
     stem_loop       1008..1051
                     /label=stem_loop
     misc_feature    997..1076
                     /label=BBa_B0010
     terminator      997..1076
                     /label=BBa_B0010
     stem_loop       1092..1111
                     /label=T7 TE
     misc_feature    1118..1118
                     /label=stop
     polyA_site      1112..1125
                     /label=polya
     misc_feature    1085..1125
                     /label=BBa_B0012
     terminator      1085..1125
                     /label=BBa_B0012
     terminator      997..1125
                     /label=BBa_B0015
     misc_feature    1..1125
                     /label=BBa_K1903010
     promoter        1134..1168
                     /label=BBa_J23101
     misc_feature    1181..1184
                     /label=conserved
     RBS             1177..1188
                     /label=BBa_B0034
     misc_feature    1195..1197
                     /label=start
     misc_feature    1657..1659
                     /label=stop
     misc_feature    1654..1656
                     /label=stop
     misc_feature    1198..1215
                     /label=tag
     misc_feature    1216..1644
                     /label=SspDnaB C intein
     misc_feature    1645..1653
                     /label=SspDnaB C extein
     misc_feature    1642..1644
                     /label=N -> A
     CDS             1195..1659
                     /label=BBa_K1362251
     stem_loop       1679..1722
                     /label=stem_loop
     misc_feature    1668..1747
                     /label=BBa_B0010
     terminator      1668..1747
                     /label=BBa_B0010
     stem_loop       1763..1782
                     /label=T7 TE
     misc_feature    1789..1789
                     /label=stop
     polyA_site      1783..1796
                     /label=polya
     misc_feature    1756..1796
                     /label=BBa_B0012
     terminator      1756..1796
                     /label=BBa_B0012
     terminator      1668..1796
                     /label=BBa_B0015
     misc_feature    1134..1796
                     /label=BBa_K1903012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ctgcgcgaaa gcggctgcat
      961 tagcggcgat agcctgatta gcctggcgta ctagagccag gcatcaaata aaacgaaagg
     1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtttacag
     1141 ctagctcagt cctaggtatt atgctagcta ctagagaaag aggagaaata ctagatgcat
     1201 catcaccatc accacagcac aggaaaaaga gtttctatta aagatttgtt agatgaaaaa
     1261 gattttgaaa tatgggcaat taatgaacag acgatgaagc tagaatcagc taaagttagt
     1321 cgtgtatttt gtactggcaa aaagctagtt tatattctaa aaactcgact aggtagaact
     1381 atcaaggcaa cagcaaatca tagattttta actattgatg gttggaaaag attagatgag
     1441 ctatctttaa aagagcatat tgctctaccc cgtaaactag aaagctcctc tttacaattg
     1501 tcaccagaaa tagaaaagtt gtctcagagt gatatttact gggactccat cgtttctatt
     1561 acggagactg gagtcgaaga ggtttttgat ttgactgtgc caggaccaca taactttgtc
     1621 gccaatgaca tcattgtcca tgcctcaggc gggtaataat actagagcca ggcatcaaat
     1681 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1741 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//