##gff-version 3
##sequence-region BBa_K1903014 1 1796
BBa_K1903014	.	engineered_region	1	1125	.	+	0	ID=BBa_K1903010;Name=BBa_K1903010
BBa_K1903014	.	CDS	941	988	.	+	0	ID=BBa_K1903019;Name=BBa_K1903019;Parent=BBa_K1903010
BBa_K1903014	.	sequence_feature	941	988	.	+	0	ID=annotation2516424;Name=Int N DnaB ;Parent=BBa_K1903019
BBa_K1903014	.	CDS	227	932	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K1903010
BBa_K1903014	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1903014	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1903014	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1903010
BBa_K1903014	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1903014	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1903014	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1903014	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1903014	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1903014	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1903014	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1903014	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1903010
BBa_K1903014	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1903014	.	terminator	997	1125	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1903010
BBa_K1903014	.	terminator	997	1076	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1903014	.	stem_loop	1008	1051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1903014	.	engineered_region	997	1076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1903014	.	terminator	1085	1125	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1903014	.	engineered_region	1085	1125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1903014	.	stop_codon	1118	1118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1903014	.	stem_loop	1092	1111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1903014	.	polyA_site	1112	1125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1903014	.	engineered_region	1134	1796	.	+	0	ID=BBa_K1903012;Name=BBa_K1903012
BBa_K1903014	.	ribosome_entry_site	1177	1188	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1903012
BBa_K1903014	.	conserved_region	1181	1184	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1903014	.	promoter	1134	1168	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1903012
BBa_K1903014	.	terminator	1668	1796	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1903012
BBa_K1903014	.	terminator	1668	1747	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1903014	.	stem_loop	1679	1722	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1903014	.	engineered_region	1668	1747	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1903014	.	terminator	1756	1796	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1903014	.	engineered_region	1756	1796	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1903014	.	stop_codon	1789	1789	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1903014	.	stem_loop	1763	1782	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1903014	.	polyA_site	1783	1796	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1903014	.	CDS	1195	1659	.	+	0	ID=BBa_K1362251;Name=BBa_K1362251;Parent=BBa_K1903012
BBa_K1903014	.	tag	1198	1215	.	+	0	ID=annotation2400724;Name=tag;Parent=BBa_K1362251
BBa_K1903014	.	stop_codon	1654	1656	.	+	0	ID=annotation2400722;Name=stop;Parent=BBa_K1362251
BBa_K1903014	.	stop_codon	1657	1659	.	+	0	ID=annotation2400719;Name=stop;Parent=BBa_K1362251
BBa_K1903014	.	sequence_alteration	1642	1644	.	+	0	ID=annotation2400753;Name=N -> A;Parent=BBa_K1362251
BBa_K1903014	.	start_codon	1195	1197	.	+	0	ID=annotation2400718;Name=start;Parent=BBa_K1362251
BBa_K1903014	.	sequence_feature	1216	1644	.	+	0	ID=annotation2400725;Name=SspDnaB C intein;Parent=BBa_K1362251
BBa_K1903014	.	sequence_feature	1645	1653	.	+	0	ID=annotation2400728;Name=SspDnaB C extein;Parent=BBa_K1362251
>BBa_K1903014
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagctgcgcga
aagcggctgcattagcggcgatagcctgattagcctggcgtactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggag
aaatactagatgcatcatcaccatcaccacagcacaggaaaaagagtttctattaaagatttgttagatgaaaaagatt
ttgaaatatgggcaattaatgaacagacgatgaagctagaatcagctaaagttagtcgtgtattttgtactggcaaaaa
gctagtttatattctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggt
tggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaattgt
caccagaaatagaaaagttgtctcagagtgatatttactgggactccatcgtttctattacggagactggagtcgaaga
ggtttttgatttgactgtgccaggaccacataactttgtcgccaatgacatcattgtccatgcctcaggcgggtaataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata