BBa_K1903014
type: ComponentDefinition
persistent identity: BBa K1903014
display id: BBa_K1903014
version: 1
was generated by: iGEM to SBOL conversion
description: Plac-RBS-RFP-IntN DnaB + PA1-RBS-IntC DnaB
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T02:20:00Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Plac-RBS-RFP-IntN DnaB + PA1-RBS + IntC DnaB
discontinued: false
dominant: false
experience: experience/None
group u list: _2369_
m user id: 29474
owner id: 29450
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Sof??a Vieto Fonseca
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1903012
component: BBa_K1903010
sequence annotation: BBa_K1903010
sequence annotation: BBa_K1903012
sequence: https://synbiohub.org/public/igem/BBa_K1903014_sequence/1

BBa_K1903012
type: Component
persistent identity: component2516382
display id: component2516382
version: 1
top level: BBa_K1903014
definition: BBa_K1903012
access: public

BBa_K1903010
type: Component
persistent identity: component2516363
display id: component2516363
version: 1
top level: BBa_K1903014
definition: BBa_K1903010
access: public

BBa_K1903010
type: SequenceAnnotation
persistent identity: annotation2516363
display id: annotation2516363
version: 1
top level: BBa_K1903014
location: https://synbiohub.org/public/igem/BBa_K1903014/annotation2516363/range2516363/1
component: BBa_K1903010

https://synbiohub.org/public/igem/BBa_K1903014/annotation2516363/range2516363/1
type: Range
persistent identity: range2516363
display id: range2516363
version: 1
top level: BBa_K1903014
direction: direction/reverse
start: 1
end: 1125
orientation: inline

BBa_K1903012
type: SequenceAnnotation
persistent identity: annotation2516382
display id: annotation2516382
version: 1
top level: BBa_K1903014
location: https://synbiohub.org/public/igem/BBa_K1903014/annotation2516382/range2516382/1
component: BBa_K1903012

https://synbiohub.org/public/igem/BBa_K1903014/annotation2516382/range2516382/1
type: Range
persistent identity: range2516382
display id: range2516382
version: 1
top level: BBa_K1903014
direction: direction/reverse
start: 1134
end: 1796
orientation: inline

BBa_K1903010
type: ComponentDefinition
persistent identity: BBa K1903010
display id: BBa_K1903010
version: 1
was generated by: iGEM to SBOL conversion
description: Plac-RBS + RFP-IntN DnaB
created: 2016-10-13T11:00:00Z
modified: 2016-10-17T08:09:10Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Plac-RBS + RFP-IntN DnaB
discontinued: false
dominant: false
experience: experience/None
group u list: _2369_
m user id: 29474
owner id: 29450
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Sof??a Vieto Fonseca
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1903019
component: BBa_E1010
component: BBa_B0034
component: BBa_R0010
component: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_K1903019
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1903010_sequence/1

BBa_K1903019
type: Component
persistent identity: component2516217
display id: component2516217
version: 1
top level: BBa_K1903010
definition: BBa_K1903019
access: public

BBa_E1010
type: Component
persistent identity: component2516216
display id: component2516216
version: 1
top level: BBa_K1903010
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2516213
display id: component2516213
version: 1
top level: BBa_K1903010
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2516205
display id: component2516205
version: 1
top level: BBa_K1903010
definition: LacI
access: public

BBa_B0015
type: Component
persistent identity: component2516224
display id: component2516224
version: 1
top level: BBa_K1903010
definition: BBa_B0015
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2516205
display id: annotation2516205
version: 1
top level: BBa_K1903010
location: https://synbiohub.org/public/igem/BBa_K1903010/annotation2516205/range2516205/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1903010/annotation2516205/range2516205/1
type: Range
persistent identity: range2516205
display id: range2516205
version: 1
top level: BBa_K1903010
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2516213
display id: annotation2516213
version: 1
top level: BBa_K1903010
location: https://synbiohub.org/public/igem/BBa_K1903010/annotation2516213/range2516213/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1903010/annotation2516213/range2516213/1
type: Range
persistent identity: range2516213
display id: range2516213
version: 1
top level: BBa_K1903010
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2516216
display id: annotation2516216
version: 1
top level: BBa_K1903010
location: https://synbiohub.org/public/igem/BBa_K1903010/annotation2516216/range2516216/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1903010/annotation2516216/range2516216/1
type: Range
persistent identity: range2516216
display id: range2516216
version: 1
top level: BBa_K1903010
direction: direction/reverse
start: 227
end: 932
orientation: inline

BBa_K1903019
type: SequenceAnnotation
persistent identity: annotation2516217
display id: annotation2516217
version: 1
top level: BBa_K1903010
location: https://synbiohub.org/public/igem/BBa_K1903010/annotation2516217/range2516217/1
component: BBa_K1903019

https://synbiohub.org/public/igem/BBa_K1903010/annotation2516217/range2516217/1
type: Range
persistent identity: range2516217
display id: range2516217
version: 1
top level: BBa_K1903010
direction: direction/reverse
start: 941
end: 988
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2516224
display id: annotation2516224
version: 1
top level: BBa_K1903010
location: https://synbiohub.org/public/igem/BBa_K1903010/annotation2516224/range2516224/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1903010/annotation2516224/range2516224/1
type: Range
persistent identity: range2516224
display id: range2516224
version: 1
top level: BBa_K1903010
direction: direction/reverse
start: 997
end: 1125
orientation: inline

BBa_K1903012
type: ComponentDefinition
persistent identity: BBa K1903012
display id: BBa_K1903012
version: 1
was generated by: iGEM to SBOL conversion
description: PA1-RBS + IntC DnaB
created: 2016-10-13T11:00:00Z
modified: 2016-10-17T08:10:16Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: PA1-RBS + IntC DnaB
discontinued: false
dominant: false
group u list: _2369_
m user id: 29474
owner id: 29450
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Sof??a Vieto Fonseca
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23101
component: BBa_K1362251
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_K1362251
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1903012_sequence/1

BBa_J23101
type: Component
persistent identity: component2516240
display id: component2516240
version: 1
top level: BBa_K1903012
definition: BBa_J23101
access: public

BBa_K1362251
type: Component
persistent identity: component2516250
display id: component2516250
version: 1
top level: BBa_K1903012
definition: BBa_K1362251
access: public

BBa_B0034
type: Component
persistent identity: component2516242
display id: component2516242
version: 1
top level: BBa_K1903012
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2516257
display id: component2516257
version: 1
top level: BBa_K1903012
definition: BBa_B0015
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2516240
display id: annotation2516240
version: 1
top level: BBa_K1903012
location: https://synbiohub.org/public/igem/BBa_K1903012/annotation2516240/range2516240/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1903012/annotation2516240/range2516240/1
type: Range
persistent identity: range2516240
display id: range2516240
version: 1
top level: BBa_K1903012
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2516242
display id: annotation2516242
version: 1
top level: BBa_K1903012
location: https://synbiohub.org/public/igem/BBa_K1903012/annotation2516242/range2516242/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1903012/annotation2516242/range2516242/1
type: Range
persistent identity: range2516242
display id: range2516242
version: 1
top level: BBa_K1903012
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1362251
type: SequenceAnnotation
persistent identity: annotation2516250
display id: annotation2516250
version: 1
top level: BBa_K1903012
location: https://synbiohub.org/public/igem/BBa_K1903012/annotation2516250/range2516250/1
component: BBa_K1362251

https://synbiohub.org/public/igem/BBa_K1903012/annotation2516250/range2516250/1
type: Range
persistent identity: range2516250
display id: range2516250
version: 1
top level: BBa_K1903012
direction: direction/reverse
start: 62
end: 526
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2516257
display id: annotation2516257
version: 1
top level: BBa_K1903012
location: https://synbiohub.org/public/igem/BBa_K1903012/annotation2516257/range2516257/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1903012/annotation2516257/range2516257/1
type: Range
persistent identity: range2516257
display id: range2516257
version: 1
top level: BBa_K1903012
direction: direction/reverse
start: 535
end: 663
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1362251
type: ComponentDefinition
persistent identity: BBa K1362251
display id: BBa_K1362251
version: 1
was generated by: iGEM to SBOL conversion
description: Intein protease
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:05Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: tag
sequence annotation: SspDnaB C intein
sequence annotation: N -> A
sequence annotation: SspDnaB C extein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1362251_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2400718
display id: annotation2400718
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400718/range2400718/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400718/range2400718/1
type: Range
persistent identity: range2400718
display id: range2400718
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 1
end: 3
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2400724
display id: annotation2400724
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400724/range2400724/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400724/range2400724/1
type: Range
persistent identity: range2400724
display id: range2400724
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 4
end: 21
orientation: inline

SspDnaB C intein
type: SequenceAnnotation
persistent identity: annotation2400725
display id: annotation2400725
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400725/range2400725/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400725/range2400725/1
type: Range
persistent identity: range2400725
display id: range2400725
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 22
end: 450
orientation: inline

N -> A
type: SequenceAnnotation
persistent identity: annotation2400753
display id: annotation2400753
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400753/range2400753/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400753/range2400753/1
type: Range
persistent identity: range2400753
display id: range2400753
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 448
end: 450
orientation: inline

SspDnaB C extein
type: SequenceAnnotation
persistent identity: annotation2400728
display id: annotation2400728
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400728/range2400728/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400728/range2400728/1
type: Range
persistent identity: range2400728
display id: range2400728
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 451
end: 459
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2400722
display id: annotation2400722
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400722/range2400722/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400722/range2400722/1
type: Range
persistent identity: range2400722
display id: range2400722
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 460
end: 462
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2400719
display id: annotation2400719
version: 1
top level: BBa_K1362251
location: https://synbiohub.org/public/igem/BBa_K1362251/annotation2400719/range2400719/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1362251/annotation2400719/range2400719/1
type: Range
persistent identity: range2400719
display id: range2400719
version: 1
top level: BBa_K1362251
direction: direction/forward
start: 463
end: 465
orientation: inline

BBa_K1903019
type: ComponentDefinition
persistent identity: BBa K1903019
display id: BBa_K1903019
version: 1
was generated by: iGEM to SBOL conversion
description: Intein N SSp DnaB S1
created: 2016-10-16T11:00:00Z
modified: 2016-10-17T08:06:56Z
mutable provenance: The sequence of amino acids: LRESGCISGDSLISLA was reverse translate to E. coli The amino acid sequence was obtained from: Volkmann, G., Volkmann, V., & Liu, X. Q. (2012). Site‐specific protein cleavage in vivo by an intein‐derived protease. FEBS letters, 586(1), 79-84.
owned by: james
owned by: myers
mutable description: This part is composed by the 11 amino acids from the SSp DnaB Intein N and 5 amino acids from the original Extein
discontinued: false
dominant: false
group u list: _2369_
m user id: 29474
owner id: 29474
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Reverse translate to the organism of interest. Needs an ATG codon to start transcription if wanted to express itself apart.
star: false
creator: Rafael Montenegro Mar??n
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Int N DnaB
sequence: https://synbiohub.org/public/igem/BBa_K1903019_sequence/1

Int N DnaB
type: SequenceAnnotation
persistent identity: annotation2516424
display id: annotation2516424
version: 1
top level: BBa_K1903019
location: https://synbiohub.org/public/igem/BBa_K1903019/annotation2516424/range2516424/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1903019/annotation2516424/range2516424/1
type: Range
persistent identity: range2516424
display id: range2516424
version: 1
top level: BBa_K1903019
direction: direction/forward
start: 1
end: 48
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1903014_sequence/1
type: Sequence
persistent identity: BBa K1903014 sequence
display id: BBa_K1903014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagctgcgcgaaagcggctgcattagcggcgatagcctgattagcctggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcatcatcaccatcaccacagcacaggaaaaagagtttctattaaagatttgttagatgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctagaatcagctaaagttagtcgtgtattttgtactggcaaaaagctagtttatattctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaattgtcaccagaaatagaaaagttgtctcagagtgatatttactgggactccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggaccacataactttgtcgccaatgacatcattgtccatgcctcaggcgggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1903010_sequence/1
type: Sequence
persistent identity: BBa K1903010 sequence
display id: BBa_K1903010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagctgcgcgaaagcggctgcattagcggcgatagcctgattagcctggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1903012_sequence/1
type: Sequence
persistent identity: BBa K1903012 sequence
display id: BBa_K1903012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcatcatcaccatcaccacagcacaggaaaaagagtttctattaaagatttgttagatgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctagaatcagctaaagttagtcgtgtattttgtactggcaaaaagctagtttatattctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaattgtcaccagaaatagaaaagttgtctcagagtgatatttactgggactccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggaccacataactttgtcgccaatgacatcattgtccatgcctcaggcgggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362251_sequence/1
type: Sequence
persistent identity: BBa K1362251 sequence
display id: BBa_K1362251_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcatcatcaccatcaccacagcacaggaaaaagagtttctattaaagatttgttagatgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctagaatcagctaaagttagtcgtgtattttgtactggcaaaaagctagtttatattctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaattgtcaccagaaatagaaaagttgtctcagagtgatatttactgggactccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggaccacataactttgtcgccaatgacatcattgtccatgcctcaggcgggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1903019_sequence/1
type: Sequence
persistent identity: BBa K1903019 sequence
display id: BBa_K1903019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgcgcgaaagcggctgcattagcggcgatagcctgattagcctggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z