LOCUS       BBa_K1903015            1672 bp    DNA     linear       26-Nov-2024
DEFINITION  PA1-RBS + GFP-linker-Quencher
ACCESSION   BBa_K1903015
VERSION     BBa_K1903015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     misc_feature    793..813
                     /label=TEV cleavage site
     CDS             790..816
                     /label=BBa_K1159318
     CDS             825..1535
                     /label=REACh1
     CDS             825..1535
                     /label=BBa_K1319001
     stem_loop       1555..1598
                     /label=stem_loop
     misc_feature    1544..1623
                     /label=BBa_B0010
     terminator      1544..1623
                     /label=BBa_B0010
     stem_loop       1639..1658
                     /label=T7 TE
     misc_feature    1665..1665
                     /label=stop
     polyA_site      1659..1672
                     /label=polya
     misc_feature    1632..1672
                     /label=BBa_B0012
     terminator      1632..1672
                     /label=BBa_B0012
     terminator      1544..1672
                     /label=BBa_B0015
     CDS             62..1672
                     /label=BBa_K1903004
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagg gagagaattt gtattttcag tcaggatact agaggtgagc aagggcgagg
      841 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      901 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      961 tcatctgcac caccggcaag ctgcccgtgc cctggcccac catcgtgacc accttcggct
     1021 acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac ttcttcaagt
     1081 ccgccatgcc cgaaggctac gaccaggagc gcaccatctt cttcaaggac gacggcaact
     1141 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1201 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactgga
     1261 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1321 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1381 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     1441 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1501 ccgccgggat cactctcggc atggacgagc tgtactacta gagccaggca tcaaataaaa
     1561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1621 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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