BBa_K1903016 1 BBa_K1903016 dCas9 A coding sequence 2016-10-16T11:00:00Z 2016-10-17T07:06:35Z The source for the Cas9 sequence was UNIPROT accession: Q99ZW2. We optimized the sequence for E. coli and changed the amino acids at positions D10A and H840A for making the protein catalytically inactive. The sequence is a coding part for a dCAs9 protein assembly with golden gate and has two windows frames for protein insertions at amino acids: L390 and E802 false false _2369_ 29474 29474 9 false This CDS was assembled with Golden Gate using four gBlocks. false Rafael Montenegro Mar??n annotation2516418 1 dCas9 A version range2516418 1 1 4134 BBa_K1903016_sequence 1 atggacaagaaatattcgattggtcttgccatcgggacgaactccgtagggtgggcggtcatcaccgacgagtacaaggtaccgtcaaaaaagtttaaagtcttgggaaatacggaccgccactcaatcaagaaaaatttgattggggccctgctgtttgattccggagagacagcagaagccacacgtttgaagcgcactgcacgccgccgctacacgcgtcgtaaaaaccgcatctgctatttgcaggaaattttctcgaacgagatggcaaaggtcgatgacagcttttttcatcgtctggaggaaagttttttggtagaagaggacaaaaagcatgaacgccaccctatcttcgggaacattgtagatgaagtcgcttaccatgagaagtatccgactatttatcatcttcgcaaaaagctggtagattctaccgataaagcggacttgcgcttgatttacttggcgctggcgcacatgatcaagttccgtggccacttcttaattgaaggtgacctgaaccccgacaattcagatgtggacaagctgttcattcaactggtacaaacctataatcagttgttcgaagaaaacccaattaatgcgtccggagtggacgcaaaggctatcttatctgcacgcttaagcaagtctcgtcgtttagagaatcttattgcacagttaccgggagagaaaaagaacggattattcggaaatcttatcgcgcttagtcttggattaacccctaatttcaaaagtaacttcgacttagctgaggatgcgaaattgcagttatccaaggacacttacgatgatgaccttgacaacctgctggcgcagattggtgatcagtacgcggacttgtttctggcggcgaaaaatttgtctgacgccattcttttgtctgatatccttcgtgttaatactgagattaccaaggcgcccctttcggcctcaatgattaaacgttacgacgagcaccatcaggacttgactcttctgaaggctcttgttcgccagcaactaccgtcttcggaaaagtacaaagagatcttcttcgatcagtccaagaacggctacgctgggtacattgacggaggcgcttcgcaagaagagttctacaagtttattaaaccgattctggagaagatggacggtacggaagagctgttagttaagctgaatcgcgaggaccgaagactgttacgcaagcaacgcacgttcgacaatggctccattccacatcaaatccatctgggtgagttacatgcaattctgcgccgccaagaggatttctatccatttcttaaagataaccgcgagaagattgaaaaaatcttgacatttcgtatcccttattatgtggggccgttagcccgtgggaacagccgcttcgcatggatgacgcgcaagtcggaagagacaatcacaccctggaattttgaggaggtagtggacaaaggcgccagcgctcaatcctttattgaacgtatgactaattttgacaaaaatttgcctaacgaaaaagtcctgccgaagcacagcctgttatatgaatactttacggtttataacgaattaacaaaagttaagtacgtgaccgagggaatgcgcaaacccgcattcctttccggcgagcaaaaaaaagcgattgtcgatcttctgttcaaaacaaaccgcaaggttacagtgaagcagcttaaggaagattactttaagaaaattgaatgtttcgattctgtcgaaatttcgggtgtggaggatcgtttcaacgcaagtcttgggacgtatcacgacttgcttaagatcatcaaagacaaggacttccttgataatgaggagaatgaggacatcttggaggacatcgtgttgaccttaactctgtttgaggatcgtgagatgatcgaagaacgccttaagacgtacgcacacttatttgacgacaaagttatgaaacagctgaagcgccgtcgctacaccgggtggggacgtttatcacgcaagcttatcaacggaatccgtgataaacaatcgggcaagacgatccttgactttctgaagtcggacggttttgcgaaccgtaatttcatgcaattaattcatgacgattctctgacatttaaagaggacatccagaaagcccaagtgtctggacagggtgacagcctgcatgaacacattgcgaacttagcgggctctccggcaattaagaagggaatcttgcaaacggtgaaggtggtggatgagttggtgaaagttatgggtcgccacaagccagaaaatattgtaatcgagatggcacgcgagaaccaaacaacccaaaagggccaaaagaacagccgtgagcgcgtcttcatgaaacgtatcgaggagggtatcaaggaattagggtcgcaaatcttgaaagaacatccagttgagaacacgcaactgcaaaacgagaaattgtacctttattatttacaaaatgggcgcgatatgtacgttgaccaagagttggatattaatcgtttgtcggactacgacgtggacgcaatgaagactgtgccacagtctttccttaaagatgattctattgataataaagtcctgacacgttctgataagaaccgtggtaagagcgacaacgtcccatctgaagaagtagtgaaaaaaatgaaaaattactggcgtcagttattaaatgcgaaattgattacgcagcgcaagttcgacaaccttactaaagctgagcgtgggggtctgtccgaactggacaaagcagggttcattaaacgccaattggtggagacacgtcaaattaccaagcatgttgcacagatcttagactctcgtatgaataccaaatacgacgaaaatgataagctgatccgtgaagtcaaagttattactcttaagtccaagctggtgtccgacttccgtaaagattttcagttctacaaggtacgcgaaatcaataactatcaccacgcccatgacgcgtatttaaatgcggtagtaggtaccgctctgatcaaaaagtatcccaaactggagagcgagttcgtttatggcgactataaagtttacgatgtacgtaagatgattgcaaaatccgaacaagaaatcgggaaagccactgcgaaatactttttctactcaaacattatgaacttctttaagacggaaattactttagccaacggcgagattcgcaaacgccctttaattgaaacaaatggcgagacgggggagattgtatgggacaaaggacgcgatttcgcaaccgttcgcaaagtacttagtatgccacaggtgaatatcgtaaagaaaacggaagtgcagactggcggcttcagcaaagaatcaatccttcccaagcgcaattcagacaagctgattgcacgcaagaaggattgggaccctaaaaagtatggcgggtttgacagccccaccgtagcgtattctgtgttagtcgtcgcaaaagtggaaaagggtaaatcgaagaagcttaaatctgtgaaagaacttttgggaatcacaattatggaacgctcttctttcgagaaaaaccctattgacttcttggaagctaaggggtacaaggaggtaaaaaaggatttgatcattaagttgcccaagtactcattattcgagctggaaaacggacgcaagcgtatgctggcctctgctggcgagttgcaaaaaggaaacgaacttgccctgccgtcaaaatacgttaacttcctgtatttggcttctcattacgaaaaactgaagggtagccctgaagataatgagcagaaacaactttttgttgagcaacataaacattatcttgatgaaattattgagcagattagcgaatttagcaaacgtgtcatcttagctgacgctaaccttgacaaagtgttaagcgcctacaacaaacatcgtgataaacctattcgtgaacaagccgagaatattatccacttgtttacactgacgaatttaggtgctccggccgcttttaagtatttcgataccaccattgatcgcaagcgctatacttctacgaaggaggtgcttgatgcaactcttattcaccaatcaatcacgggtctgtatgaaacgcgcattgatttgagtcagttgggcggtgattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z