BBa_K1903018 1 BBa_K1903018 Golden Gate Adapter 2016-10-16T11:00:00Z 2016-10-19T10:00:51Z The restriction site sequence was obtained from NEB enzyme finder This parts is composed of a random sequence of 100 base pairs follow upstream and downstream by a BsaI restriction site. The fusion sites for both ends are part of the standard prefix and the suffix. The part has the advantage to be clone into a RFP plasmid with RFC 10 and then used as the vector for the Golden Gate reaction. true false _2369_ 29474 29474 9 false We had a random sequence into the BsaI cut sites to make a sequence easy to detect after a PCR reaction and leading the enzyme digestion. For further design a good option is to change the random sequence with a RFP o GFP device, using J23101 for example, to have a selection in the transformation. false Rafael Montenegro Mar??n annotation2516419 1 Golden Gate Adapter range2516419 1 1 113 BBa_K1903018_sequence 1 gagacccttatcatatttgttttgacgactgccgtcttcgctgtttctttagacatttaacaataaacgctttttgtaggcagaggtaccccctattagtggctgcggtctca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z