BBa_K1903018
1
BBa_K1903018
Golden Gate Adapter
2016-10-16T11:00:00Z
2016-10-19T10:00:51Z
The restriction site sequence was obtained from NEB enzyme finder
This parts is composed of a random sequence of 100 base pairs follow upstream and downstream by a BsaI restriction site. The fusion sites for both ends are part of the standard prefix and the suffix. The part has the advantage to be clone into a RFP plasmid with RFC 10 and then used as the vector for the Golden Gate reaction.
true
false
_2369_
29474
29474
9
false
We had a random sequence into the BsaI cut sites to make a sequence easy to detect after a PCR reaction and leading the enzyme digestion. For further design a good option is to change the random sequence with a RFP o GFP device, using J23101 for example, to have a selection in the transformation.
false
Rafael Montenegro Mar??n
annotation2516419
1
Golden Gate Adapter
range2516419
1
1
113
BBa_K1903018_sequence
1
gagacccttatcatatttgttttgacgactgccgtcttcgctgtttctttagacatttaacaataaacgctttttgtaggcagaggtaccccctattagtggctgcggtctca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z