BBa_K1904002 1 BBa_K1904002 Mutated endolysin specific for Acinetobacter baumannii with terminator. C-terminal HisTag 2016-06-06T11:00:00Z 2016-06-07T09:29:21Z Acinetobacter baumannii phage Abp1. This biobrick is composed by the mutated endolysin (BBa_K1904000) assembled with a double terminator (B0015), BBa_K1904002 is in RFC 25 standard to allow protein engineering. As the part does not contain a promoter it should be cloned into a promoter-containing plasmid for its usage. Additionally the part was complemented with a C-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography. false false _2370_ 25405 29496 9 false This sequence was optimised for its usage in RFC 25 standard and in E. coli as chassis. false Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo annotation2480038 1 His Tag range2480038 1 553 570 annotation2480039 1 B0015 range2480039 1 571 702 annotation2480037 1 K1904000 range2480037 1 1 552 BBa_K1904002_sequence 1 attttaacaaaggatgggttttcaatcatccgcaatgaattgtttgggggaaagttggatcagacacaggttgacgccatcaacttcatcgtagaaaaagcaacggagagcggcttatcctatcctgaggcagcatacttattagccaccatctaccatgagacgggccttccttccgggtatcgtactatgcaacccatcaaggaagctgggtcagattcttatttgcgctctaagaaatattatccgtacattggttatggatatgttcaattgacgtgggaggagaattacgggcgtatctcaaaattgattggcgtggatttgatcaagaacccagagaaggctttagaacccttaatcgcgatccaaattgcgattaaaggcatgcttaatgggtggtttactggggtcggttttcgccgtaagcgcccagtctccaagtataataaacaacaatatgtggctgctcgtaacattattaacggtaaagacaaagccgagttgattgcgaagtacgctattatctttgaacgcgcattacgcagtttacatcaccatcaccatcactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z