BBa_K1904003 1 BBa_K1904003 T4 Holin with RBS and terminator. 2016-06-06T11:00:00Z 2016-06-07T05:41:11Z T4 bacteriophage This biobrick is contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS) and a double terminator (B0015). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. false false _2370_ 25405 29496 9 false This biobrick was optimised for its usage in RFC10 standard and in E. coli. false Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo annotation2480007 1 scar range2480007 1 13 18 annotation2480006 1 B0034 range2480006 1 1 12 annotation2480008 1 T4 Holin range2480008 1 19 675 annotation2480009 1 B0015 range2480009 1 676 804 BBa_K1904003_sequence 1 aaagaggagaaatactagatggctgcaccccgtatctcattctcaccgtccgacatcttattcggagttttagatagacttttcaaagacaacgcaacagggaaggtgttggcgagccgtgtggcggtcgtcatccttcttttcataatggctatcgtatggtatcggggggactccttctttgaatactataaacaatctaaatacgaaacctattcagaaataatagagaaggagagaacagctagattcgaaagcgtggcacttgaacaactgcaaattgtccacatttcatctgaggccgatttttccgcagtctattcatttcgtccaaaaaatttgaactacttcgtagatattatcgcatacgaaggcaaattacctagtaccataagtgaaaagtcactgggaggttaccctgtagacaagactatggatgaatacactgttcatctgaatgggcgtcattattactctaattctaagttcgcctttctgccaaccaagaaaccgactcctgagatcaactatatgtactcgtgtccttacttcaaccttgataatatatatgctgggacgatcaccatgtattggtatcggaacgaccatatttcgaacgaccgccttgaatccatttgcgcccaggctgcacgtatccttggacgcgccaaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z