BBa_K1904004 1 BBa_K1904004 T4 Holin with RBS (RFC 25 N-Part). 2016-06-06T11:00:00Z 2016-06-07T06:25:47Z T4 bacteriophage This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the holin is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. false false _2370_ 29496 29496 9 false This biobrick was optimised for its usage in RFC 25 standard and in E. coli, as chasis. It is designed as an N-part and it does not contain restriction site for NgoMIV. false Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo annotation2480042 1 T4 Holin range2480042 1 19 672 annotation2480040 1 B0034 range2480040 1 1 12 annotation2480041 1 scar range2480041 1 13 18 BBa_K1904004_sequence 1 aaagaggagaaatactagatggctgcaccccgtatctcattctcaccgtccgacatcttattcggagttttagatagacttttcaaagacaacgcaacagggaaggtgttggcgagccgtgtggcggtcgtcatccttcttttcataatggctatcgtatggtatcggggggactccttctttgaatactataaacaatctaaatacgaaacctattcagaaataatagagaaggagagaacagctagattcgaaagcgtggcacttgaacaactgcaaattgtccacatttcatctgaggccgatttttccgcagtctattcatttcgtccaaaaaatttgaactacttcgtagatattatcgcatacgaaggcaaattacctagtaccataagtgaaaagtcactgggaggttaccctgtagacaagactatggatgaatacactgttcatctgaatgggcgtcattattactctaattctaagttcgcctttctgccaaccaagaaaccgactcctgagatcaactatatgtactcgtgtccttacttcaaccttgataatatatatgctgggacgatcaccatgtattggtatcggaacgaccatatttcgaacgaccgccttgaatccatttgcgcccaggctgcacgtatccttggacgcgccaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z