BBa_K1904005 1 BBa_K1904005 Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part). 2016-06-06T11:00:00Z 2016-06-07T06:26:20Z Acinetobacter baumannii phage Abp1 and bacteriophage T4 This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography. false false _2370_ 29496 29496 9 false This sequence was optimised for its usage in RFC 25 standard and in E. coli as chassis. It is designed as a Freiburg N-part and it does not contain restriction site for NgoMIV. false Enrique Chimal Juarez, Roger Milton Rubio S??nchez, Enrique Palomino, Brian Morteo annotation2480050 1 B0015 range2480050 1 1252 1380 annotation2480044 1 scar range2480044 1 13 18 annotation2480046 1 scar range2480046 1 673 678 annotation2480043 1 B0034 range2480043 1 1 12 annotation2480045 1 K1904004 range2480045 1 19 672 annotation2480049 1 stop range2480049 1 1249 1251 annotation2480047 1 K1904002 range2480047 1 679 1230 annotation2480048 1 His Tag range2480048 1 1230 1248 BBa_K1904005_sequence 1 aaagaggagaaatactagatggctgcaccccgtatctcattctcaccgtccgacatcttattcggagttttagatagacttttcaaagacaacgcaacagggaaggtgttggcgagccgtgtggcggtcgtcatccttcttttcataatggctatcgtatggtatcggggggactccttctttgaatactataaacaatctaaatacgaaacctattcagaaataatagagaaggagagaacagctagattcgaaagcgtggcacttgaacaactgcaaattgtccacatttcatctgaggccgatttttccgcagtctattcatttcgtccaaaaaatttgaactacttcgtagatattatcgcatacgaaggcaaattacctagtaccataagtgaaaagtcactgggaggttaccctgtagacaagactatggatgaatacactgttcatctgaatgggcgtcattattactctaattctaagttcgcctttctgccaaccaagaaaccgactcctgagatcaactatatgtactcgtgtccttacttcaaccttgataatatatatgctgggacgatcaccatgtattggtatcggaacgaccatatttcgaacgaccgccttgaatccatttgcgcccaggctgcacgtatccttggacgcgccaaaaccggcattttaacaaaggatgggttttcaatcatccgcaatgaattgtttgggggaaagttggatcagacacaggttgacgccatcaacttcatcgtagaaaaagcaacggagagcggcttatcctatcctgaggcagcatacttattagccaccatctaccatgagacgggccttccttccgggtatcgtactatgcaacccatcaaggaagctgggtcagattcttatttgcgctctaagaaatattatccgtacattggttatggatatgttcaattgacgtgggaggagaattacgggcgtatctcaaaattgattggcgtggatttgatcaagaacccagagaaggctttagaacccttaatcgcgatccaaattgcgattaaaggcatgcttaatgggtggtttactggggtcggttttcgccgtaagcgcccagtctccaagtataataaacaacaatatgtggctgctcgtaacattattaacggtaaagacaaagccgagttgattgcgaagtacgctattatctttgaacgcgcattacgcagtttacatcaccatcaccatcactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z