BBa_K1905003 1 BBa_K1905003 Riboswitch to detect E5 mRNA from HPV-16 and express RFP 2016-09-12T11:00:00Z 2016-09-13T10:11:39Z It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E5 mRNA and code RFP. DNA coding for a riboswitch to detect E5 mRNA from Human Papillomavirus type 16 and express Red Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E5 DNA coding region, and the original sequence with one base changed, RFP coding region (BBa_E1010) and a terminator (BBa_B0015). When there is presence of E5 mRNA, the riboswitch turns on and it allows the expression of RFP as reporter protein. false false _2371_ 26350 26350 9 false The main design consideration is the proper fold of the mRNA and hybridization with E5 mRNA. false Juan Carlos Rueda Silva annotation2483296 1 BBa_B0015 range2483296 1 998 1126 annotation2483290 1 BBa_B0010 range2483290 1 998 1077 annotation2483291 1 stem_loop range2483291 1 1009 1052 annotation2483286 1 BBa_K1905000 range2483286 1 1 277 annotation2483295 1 stop range2483295 1 1119 1119 annotation2483294 1 polya range2483294 1 1113 1126 annotation2483289 1 BBa_E1010 range2483289 1 284 989 annotation2483288 1 Help:Barcodes range2483288 1 965 989 annotation2483293 1 T7 TE range2483293 1 1093 1112 annotation2483292 1 BBa_B0012 range2483292 1 1086 1126 annotation2483287 1 mrfp1 range2483287 1 284 958 BBa_K1905003_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatgttaacctaaacgcagaggctgcaagcacagagaaaagaggagaaaaagcacagagagcagcctctgcgtataggttactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z