BBa_K1905004 1 BBa_K1905004 Riboswitch to detect L1 mRNA from HPV and express BFP 2016-09-12T11:00:00Z 2016-09-13T09:55:42Z It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of L1 mRNA and code BFP. DNA coding for a riboswitch to detect L1 mRNA from Human Papillomavirus and express Blue Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of L1 DNA coding region, and the original sequence with one base changed, BFP coding region (BBa_K592009) and a terminator (BBa_B0015). When there is presence of L1 mRNA, the riboswitch turns on and it allows the expression of BFP as reporter protein. false false _2371_ 26350 26350 9 false The main design consideration is the proper fold of the mRNA and hybridization with L1 mRNA. false Juan Carlos Rueda Silva annotation2483301 1 stem_loop range2483301 1 978 1021 annotation2483302 1 BBa_B0012 range2483302 1 1055 1095 annotation2483303 1 T7 TE range2483303 1 1062 1081 annotation2483306 1 BBa_B0015 range2483306 1 967 1095 annotation2483300 1 BBa_B0010 range2483300 1 967 1046 annotation2483298 1 amilCP range2483298 1 290 955 annotation2483299 1 BBa_K592009 range2483299 1 290 958 annotation2483297 1 BBa_K1905001 range2483297 1 1 283 annotation2483304 1 polya range2483304 1 1082 1095 annotation2483305 1 stop range2483305 1 1088 1088 BBa_K1905004_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacagaaaaccaaacttattggggtcaggtaaagcacagagaaaagaggagaaaaagcacagagatacctgaccgcaataagtttggtactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z