BBa_K1905005 1 BBa_K1905005 Riboswitch to detect E6 mRNA from HPV and express YFP 2016-09-12T11:00:00Z 2016-09-13T10:03:08Z It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E6 mRNA and code YFP. DNA coding for a riboswitch to detect E6 mRNA from Human Papillomavirus type 16 and type 18 and express Yellow Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E6 DNA coding region, and the original sequence with one base changed, YFP coding region (BBa_K592010) and a terminator (BBa_B0015). When there is presence of E6 mRNA, the riboswitch turns on and it allows the expression of YFP as reporter protein. false false _2371_ 26350 26350 9 false The main design consideration is the proper fold of the mRNA and hybridization with E6 mRNA false Juan Carlos Rueda Silva BBa_K1905005_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacattttagaataaaactttaaacatttataagcacagagaaaagaggagaaaaagcacagagaataaatgtttaaagttatattctactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z