LOCUS       BBa_K1908000            3039 bp    DNA     linear       22-Dec-2024
DEFINITION  Blue-Yellow fluorescent protein toggle switch using pLac and OmpC 
            promoter.
ACCESSION   BBa_K1908000
VERSION     BBa_K1908000.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..75
                     /label=BBa_J61100
     CDS             82..792
                     /label=fwYellow
     CDS             82..795
                     /label=BBa_K1033910
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     terminator      804..932
                     /label=BBa_B0015
     misc_feature    953..970
                     /label=C1 OmpR
     misc_feature    974..991
                     /label=C2 OmpR
     misc_feature    994..1011
                     /label=C3 OmpR
     promoter        1015..1020
                     /label=-35
     promoter        1038..1043
                     /label=-10
     promoter        941..1048
                     /label=BBa_R0082
     RBS             1057..1068
                     /label=BBa_J61100
     CDS             1075..1740
                     /label=amilCP
     CDS             1075..1743
                     /label=BBa_K592009
     misc_feature    2839..2877
                     /label=LVA
     CDS             1750..2877
                     /label=lacI-LVA
     misc_feature    1750..2877
                     /label=BBa_C0012
     misc_feature    2878..2902
                     /label=Help:Barcodes
     CDS             1750..2877
                     /label=BBa_C0012
     stem_loop       2922..2965
                     /label=stem_loop
     misc_feature    2911..2990
                     /label=BBa_B0010
     terminator      2911..2990
                     /label=BBa_B0010
     stem_loop       3006..3025
                     /label=T7 TE
     misc_feature    3032..3032
                     /label=stop
     polyA_site      3026..3039
                     /label=polya
     misc_feature    2999..3039
                     /label=BBa_B0012
     terminator      2999..3039
                     /label=BBa_B0012
     terminator      2911..3039
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg ggacatacta gatgacggca ctgactgaag gcgcaaaact gttcgagaaa
      121 gaaatcccat atatcactga gctggaaggt gacgttgaag gtatgaagtt tatcatcaag
      181 ggtgaaggta ccggtgacgc gagcgtcggt aaagtggatg ctcagttcat ttgtaccacg
      241 ggcgacgttc cggttccgtg gagcacgctg gtcaccacgc tgacgtatgg tgctcagtgc
      301 tttgccaagt atccgcgcca cattgcggat ttcttcaaaa gctgcatgcc ggaaggttac
      361 gtccaagagc gcaccatcac ctttgagggt gatggcgtgt tcaagacccg tgcggaagtc
      421 acctttgaaa atggcagcgt gtacaaccgt gtaaaactga acggccaggg tttcaagaag
      481 gacggccacg tgctgggcaa aaatctggag tttaacttta cccctcattg tttgtacatt
      541 tggggtgacc aagcgaatca tggcctgaag agcgcgttca aaatcatgca tgagatcacc
      601 ggctccaaag aggatttcat tgttgccgat cacacccaaa tgaatacccc gattggtggt
      661 ggtccggtgc acgtgccgga gtaccaccac attacgtatc atgttaccct gtctaaagac
      721 gtcaccgatc accgtgacca tttgaacatt gttgaggtga tcaaggcagt tgacctggag
      781 acgtaccgtt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcccttgcat ttacattttg
      961 aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt cgtgttggat
     1021 tattctgcat ttttggggag aatggactta ctagagaaag aggggacata ctagatgagt
     1081 gtgatcgcta aacaaatgac ctacaaggtt tatatgtcag gcacggtcaa tggacactac
     1141 tttgaggtcg aaggcgatgg aaaaggtaag ccctacgagg gggagcagac ggtaaagctc
     1201 actgtcacca agggcggacc tctgccattt gcttgggata ttttatcacc acagtgtcag
     1261 tacggaagca taccattcac caagtaccct gaagacatcc ctgactatgt aaagcagtca
     1321 ttcccggagg gctatacatg ggagaggatc atgaactttg aagatggtgc agtgtgtact
     1381 gtcagcaatg attccagcat ccaaggcaac tgtttcatct accatgtcaa gttctctggt
     1441 ttgaactttc ctcccaatgg acctgtcatg cagaagaaga cacagggctg ggaacccaac
     1501 actgagcgtc tctttgcacg agatggaatg ctgctaggaa acaactttat ggctctgaag
     1561 ttagaaggag gcggtcacta tttgtgtgaa tttaaaacta cttacaaggc aaagaagcct
     1621 gtgaagatgc cagggtatca ctatgttgac cgcaaactgg atgtaaccaa tcacaacaag
     1681 gattacactt cggttgagca gtgtgaaatt tccattgcac gcaaacctgt ggtcgcctaa
     1741 taatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     1801 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     1861 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     1921 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     1981 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     2041 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     2101 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     2161 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     2221 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     2281 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     2341 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     2401 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     2461 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     2521 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     2581 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     2641 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     2701 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     2761 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2821 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
     2881 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt
     2941 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3001 acactggctc accttcgggt gggcctttct gcgtttata
//