BBa_K1909001 1 BBa_K1909001 pathogen associated molecular pattern sensor 2016-10-12T11:00:00Z 2016-10-13T02:23:39Z EnvZ - http://www.uniprot.org/uniprot/P0AEJ4 NOD1 - http://www.uniprot.org/uniprot/Q9Y239 We created a fusion protein, joining the EnvZ two-component system signalling domain to the sensing leucine-rich repeat domain of the pattern recognition receptor NOD1. The EnvZ kinase domain autophosphorylates after target binding and activates OmpR, a prokaryotic selective transcription factor. Therefore it is able to sense pathogen associated molecular patterns (e.g. LPS, mDAP) and subsequently activate reporter expression. false false _2375_ 29614 29614 9 false To prevent premature ribosome drop-off, the pair of CTA codons (L77 and L79 of the LRR domain) predicted to strongly stall the translation was substituted by TTA codons. An XbaI restriction site was removed by changing L239 from CTA to TTA. To make up for the lost translational pause, L242 was changed from CTG to CTA (Fig 1C). We also added an eight aminoacid FLAG-tag at residue 16, allowing for expression confirmation by targeted purification. false Daniel Cort??s annotation2496582 1 FLAG range2496582 1 46 69 annotation2496586 1 EnvZ Kinase domain range2496586 1 1136 2011 annotation2496584 1 NOD-LRR domain range2496584 1 169 1135 BBa_K1909001_sequence 1 atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacggattataaagatgatgatgataaattattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttggcgccctgaagagcctgccccgcgttcaggtcgaaagcttcaaccaggtgcaggccatgcccacgttcatctggatgctgcgctgcatctacgagacacagagccagaaggtggggcagctggcggccaggggcatctgcgccaactacctcaagctgacctactgcaacgcctgctcggccgattgcagcgccctctccttcgtcctgcatcacttccccaagcggctggccttagacttagacaacaacaatctcaacgactacggcgtgcgggagttgcagccctgcttcagccgcctcactgttctcagactcagcgtaaaccagatcactgacggtggggtaaaggtgctaagcgaagagctgaccaaatacaaaattgtgacctatttgggtttatacaacaaccagatcaccgatgtcggagccaggtacgtcaccaaaatcctggatgaatgcaaaggcctcacgcatcttaaactgggaaaaaacaaaataacaagtgaaggagggaagtatctcgccctggctgtgaagaacagcaaatcaatctctgaggttgggatgtggggcaatcaagttggggatgaaggagcaaaagccttcgcagaggctctgcggaaccaccccagcttgaccaccctgagtcttgcgtccaacggcatctccacagaaggaggaaagagccttgcgagggccttgcagcagaacacgtctttagaaatactatggctgacccaaaatgaactcaacgatgaagtggcagagagtttggcagaaatgttgaaagtcaaccagacgttaaagcatttatggcttatccagaatcagatcacagctaaggggactgcccagctggcagatgcgttacagagcaacactggcataacagagatttgcctaaatggaaacctgataaaaccagaggaggccaaagtctatgaagatgagaagcggattatctgtttctctcgctatacgctggcgattatgctattggcgatcggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaataaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z