LOCUS       BBa_K191005             1864 bp    DNA     linear       29-Nov-2024
DEFINITION  TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term
ACCESSION   BBa_K191005
VERSION     BBa_K191005.1  
FEATURES             Location/Qualifiers
     promoter        1..85
                     /label=BBa_K191007
     misc_feature    98..101
                     /label=conserved
     RBS             94..105
                     /label=BBa_B0034
     CDS             115..731
                     /label=tetR
     misc_feature    732..765
                     /label=SsrA
     misc_feature    772..796
                     /label=Help:Barcodes
     CDS             112..796
                     /label=BBa_C0040
     stem_loop       816..859
                     /label=stem_loop
     misc_feature    805..884
                     /label=BBa_B0010
     terminator      805..884
                     /label=BBa_B0010
     stem_loop       900..919
                     /label=T7 TE
     misc_feature    926..926
                     /label=stop
     polyA_site      920..933
                     /label=polya
     misc_feature    893..933
                     /label=BBa_B0012
     terminator      893..933
                     /label=BBa_B0012
     misc_feature    942..960
                     /label=TetR 1
     misc_feature    942..995
                     /label=BBa_R0040
     promoter        961..966
                     /label=-35
     misc_feature    967..985
                     /label=TetR 2
     promoter        984..989
                     /label=-10
     promoter        942..995
                     /label=BBa_R0040
     misc_feature    1008..1011
                     /label=conserved
     RBS             1004..1015
                     /label=BBa_B0034
     CDS             1022..1696
                     /label=mrfp1
     misc_feature    1703..1727
                     /label=Help:Barcodes
     CDS             1022..1702
                     /label=BBa_E1010
     stem_loop       1747..1790
                     /label=stem_loop
     misc_feature    1736..1815
                     /label=BBa_B0010
     terminator      1736..1815
                     /label=BBa_B0010
     stem_loop       1831..1850
                     /label=T7 TE
     misc_feature    1857..1857
                     /label=stop
     polyA_site      1851..1864
                     /label=polya
     misc_feature    1824..1864
                     /label=BBa_B0012
     terminator      1824..1864
                     /label=BBa_B0012
     misc_feature    1004..1864
                     /label=BBa_I13507
ORIGIN
        1 tggcaaatat tctgaaatga gctgttgaca attaatcatc gaactagtta actagtacgc
       61 aagttcacgt aaaaagggta tcgactacta gagaaagagg agaaatacta gatgtccaga
      121 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
      181 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
      241 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
      301 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
      361 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
      421 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
      481 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
      541 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
      601 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
      661 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
      721 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag
      781 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      901 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga
      961 ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta
     1021 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1081 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     1141 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     1201 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     1261 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     1321 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     1381 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     1441 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     1501 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1561 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     1621 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     1681 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
     1741 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1801 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1861 tata
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