LOCUS       BBa_K191008             1053 bp    DNA     linear       04-Jul-2024
DEFINITION  Promoter LacI - RBS - LovTAP(ILE427PHE) - Term
ACCESSION   BBa_K191008
VERSION     BBa_K191008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..916
                     /label=BBa_K191009
     stem_loop       936..979
                     /label=stem_loop
     misc_feature    925..1004
                     /label=BBa_B0010
     terminator      925..1004
                     /label=BBa_B0010
     stem_loop       1020..1039
                     /label=T7 TE
     misc_feature    1046..1046
                     /label=stop
     polyA_site      1040..1053
                     /label=polya
     misc_feature    1013..1053
                     /label=BBa_B0012
     terminator      1013..1053
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgttggctac
      241 tacacttgaa cgtattgaga agaactttgt cattactgac ccaaggttgc cagataatcc
      301 ctttatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt
      361 gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat
      421 tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag
      481 tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt
      541 ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag
      601 agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg catttgtcga
      661 cctgcttaag aatgcctacc aaaacgatct ccatttaccg ttgttaaacc tgatgctgac
      721 gccagatgag cgcgaagcgt tggggactcg cgtgcgtatt gtcgaagagc tgttgcgcgg
      781 cgaaatgagc cagcgtgagt taaaaaatga actcggcgcg ggcatcgcga cgattacgcg
      841 tggatctaac agcctgaaag ccgcgcccgt tgagctgcgc cagtggctgg aagaggtgtt
      901 gctgaaaagc gattgatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      961 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1021 gctcaccttc gggtgggcct ttctgcgttt ata
//