##gff-version 3 ##sequence-region BBa_K191008 1 1053 BBa_K191008 . ribosome_entry_site 209 223 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_K191008 . ribosome_entry_site 209 223 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K191008 . engineered_region 209 223 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K191008 . ribosome_entry_site 216 220 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K191008 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K191008 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K191008 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K191008 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K191008 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K191008 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K191008 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K191008 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K191008 . CDS 230 916 . + 0 ID=BBa_K191009;Name=BBa_K191009 BBa_K191008 . terminator 1013 1053 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K191008 . engineered_region 1013 1053 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K191008 . stop_codon 1046 1046 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K191008 . polyA_site 1040 1053 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K191008 . stem_loop 1020 1039 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K191008 . terminator 925 1004 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K191008 . engineered_region 925 1004 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K191008 . stem_loop 936 979 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 >BBa_K191008 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgttggc tactacacttgaacgtattgagaagaactttgtcattactgacccaaggttgccagataatccctttatattcgcgtcc gatagtttcttgcagttgacagaatatagccgtgaagaaattttgggaagaaactgcaggtttctacaaggtcctgaaa ctgatcgcgcgacagtgagaaaaattagagatgccatagataaccaaacagaggtcactgttcagctgattaattatac aaagagtggtaaaaagttctggaacctctttcacttgcagcctatgcgagatcagaagggagatgtccagtactttatt ggggttcagttggatggaactgagcatgtccgagatgctgccgagagagagggagtcatgctgattaagaaaactgcag aaaatattgatgaggcggcatttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacct gatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagc cagcgtgagttaaaaaatgaactcggcgcgggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccg ttgagctgcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaagg ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc ttcgggtgggcctttctgcgtttata