BBa_K1911003 1 BBa_K1911003 ClpX from E. Coli 2016-10-08T11:00:00Z 2016-10-15T11:01:10Z ClpX is found naturally in the E. coli genome. ClpX is part of the protein-degradation system called ClpXP. It works in unison with another protein called ClpXP so that it can de-linearize and unfold proteins into amino acids which can then be recycled by the cells. In E. coli cells, ClpXP focuses on degrading incorrect proteins and reducing them down into amino acids which can be recycled by the cells. ClpXP recognizes the incorrect proteins by certain SsrA-tags which are attached at the ends of incorrect proteins. ClpX in unison with ClpP can be used to degrade unwanted proteins and can be used in future projects in conjunction with certain degradation tags. false false _2378_ 28764 28764 9 false There were illegal restriction sites which cut the ClpX gene into smaller sequences, rendering it obsolete. So we had to introduce wobbles to eliminate these illegal restriction sites so that ClpX may be expressed and allowed to function as a complete protein. false Jack Kwon BBa_K1911003_sequence 1 atgacagataaacgcaaagatggctcaggcaaattgctgtattgctctttttgcggcaaaagccagcatgaagtgcgcaagctgattgccggtccatccgtgtatatctgcgacgaatgtgttgatttatgtaacgacatcattcgcgaagagattaaagaagttgcaccgcatcgtgaacgcagtgcgctaccgacgccgcatgaaattcgcaaccacctggacgattacgttatcggccaggaacaggcgaaaaaagtgctggcggtcgcggtatacaaccattacaaacgtctgcgcaacggcgataccagcaatggcgtcgagttgggcaaaagtaacattctgctgatcggtccgaccggttccggtaaaacgctgctggctgaaacgctggcgcgcctgctggatgttccgttcaccatggccgacgcgactacactgaccgaagccggttatgtgggtgaagacgttgaaaacatcattcagaagctgttgcagaaatgcgactacgatgtccagaaagcacagcgtggtattgtctacatcgatgaaatcgacaagatttctcgtaagtcagacaacccgtccattacccgagacgtttccggtgaaggcgtacagcaggcactgttgaaactgatcgaaggtacggtagctgctgttccaccgcaaggtgggcgtaaacatccgcagcaggagttcttgcaggttgatacctctaagatcctgtttatttgtggcggtgcgtttgccggtctggataaagtgatttcccaccgtgtagaaaccggctccggcattggttttggcgcgacggtaaaagcgaagtccgacaaagcaagcgaaggcgagctgctggcgcaggttgaaccggaagatctgatcaagtttggtcttatccctgagtttattggtcgtctgccggttgtcgcaacgttgaatgaactgagcgaagaagctctgattcagatcctcaaagagccgaaaaacgccctgaccaagcagtatcaggcgctgtttaatctggaaggcgtggatctggagttccgtgacgaggcgctggatgctatcgctaagaaagcgatggcgcgtaaaaccggtgcccgtggcctgcgttccatcgtagaagccgcactgctcgataccatgtacgatctgccgtccatggaagacgtcgaaaaagtggttatcgacgagtcggtaattgatggtcaaagcaaaccgttgctgatttatggcaagccggaagcgcaacaggcatctggtgaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z