##gff-version 3
##sequence-region BBa_K1911004 1 4960
BBa_K1911004	.	ribosome_entry_site	4169	4179	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	4169	4179	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911004	.	engineered_region	4169	4179	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	4175	4178	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911004	.	promoter	2157	2210	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1911004	.	operator	2159	2175	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1911004	.	promoter	2176	2181	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1911004	.	promoter	2199	2204	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1911004	.	operator	2182	2198	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1911004	.	engineered_region	2157	2210	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1911004	.	ribosome_entry_site	58	68	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	58	68	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911004	.	engineered_region	58	68	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	1236	1247	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1911004	.	conserved_region	1240	1243	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1911004	.	CDS	1254	1970	.	+	0	ID=BBa_K1911005;Name=BBa_K1911005
BBa_K1911004	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1911004	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1911004	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1911004	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1911004	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1911004	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1911004	.	tag	1979	2011	.	+	0	ID=BBa_M0050;Name=BBa_M0050
BBa_K1911004	.	tag	1979	2011	.	+	0	ID=annotation1958880;Name=WT SsrA tag AANDENYALAA;Parent=BBa_M0050
BBa_K1911004	.	CDS	4186	4960	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K1911004	.	tag	4897	4929	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1911004	.	CDS	4189	4896	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1911004	.	engineered_tag	4936	4960	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1911004	.	CDS	3537	4160	.	+	0	ID=BBa_K1911002;Name=BBa_K1911002
BBa_K1911004	.	CDS	2237	3511	.	+	0	ID=BBa_K1911003;Name=BBa_K1911003
BBa_K1911004	.	ribosome_entry_site	3520	3530	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	3520	3530	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911004	.	engineered_region	3520	3530	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	3526	3529	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	2220	2230	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	2220	2230	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911004	.	engineered_region	2220	2230	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911004	.	ribosome_entry_site	2226	2229	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911004	.	terminator	2020	2148	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1911004	.	terminator	2108	2148	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1911004	.	polyA_site	2135	2148	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1911004	.	stem_loop	2115	2134	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1911004	.	engineered_region	2108	2148	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1911004	.	stop_codon	2141	2141	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1911004	.	terminator	2020	2099	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1911004	.	stem_loop	2031	2074	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1911004	.	engineered_region	2020	2099	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1911004	.	CDS	75	1202	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1911004	.	engineered_region	75	1202	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1911004	.	CDS	75	1202	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1911004	.	engineered_tag	1203	1227	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1911004	.	tag	1164	1202	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_K1911004
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggactactagatggt
gaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaac
caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcg
tggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgca
aattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtc
gaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgacc
aggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaa
cagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcg
ctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa
ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgaggg
catcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctg
cgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacca
tcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaaggg
caatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcg
ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacg
ctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggctagcaa
aggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttc
catggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggca
acattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat
caaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca
attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa
agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattacta
gaggctgctaacgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacat
actagagtcacacaggactactagatgacagataaacgcaaagatggctcaggcaaattgctgtattgctctttttgcg
gcaaaagccagcatgaagtgcgcaagctgattgccggtccatccgtgtatatctgcgacgaatgtgttgatttatgtaa
cgacatcattcgcgaagagattaaagaagttgcaccgcatcgtgaacgcagtgcgctaccgacgccgcatgaaattcgc
aaccacctggacgattacgttatcggccaggaacaggcgaaaaaagtgctggcggtcgcggtatacaaccattacaaac
gtctgcgcaacggcgataccagcaatggcgtcgagttgggcaaaagtaacattctgctgatcggtccgaccggttccgg
taaaacgctgctggctgaaacgctggcgcgcctgctggatgttccgttcaccatggccgacgcgactacactgaccgaa
gccggttatgtgggtgaagacgttgaaaacatcattcagaagctgttgcagaaatgcgactacgatgtccagaaagcac
agcgtggtattgtctacatcgatgaaatcgacaagatttctcgtaagtcagacaacccgtccattacccgagacgtttc
cggtgaaggcgtacagcaggcactgttgaaactgatcgaaggtacggtagctgctgttccaccgcaaggtgggcgtaaa
catccgcagcaggagttcttgcaggttgatacctctaagatcctgtttatttgtggcggtgcgtttgccggtctggata
aagtgatttcccaccgtgtagaaaccggctccggcattggttttggcgcgacggtaaaagcgaagtccgacaaagcaag
cgaaggcgagctgctggcgcaggttgaaccggaagatctgatcaagtttggtcttatccctgagtttattggtcgtctg
ccggttgtcgcaacgttgaatgaactgagcgaagaagctctgattcagatcctcaaagagccgaaaaacgccctgacca
agcagtatcaggcgctgtttaatctggaaggcgtggatctggagttccgtgacgaggcgctggatgctatcgctaagaa
agcgatggcgcgtaaaaccggtgcccgtggcctgcgttccatcgtagaagccgcactgctcgataccatgtacgatctg
ccgtccatggaagacgtcgaaaaagtggttatcgacgagtcggtaattgatggtcaaagcaaaccgttgctgatttatg
gcaagccggaagcgcaacaggcatctggtgaataatactagagtcacacaggactactagatgtcatacagcggcgaac
gagataactttgcaccccatatggcgctggtgccgatggtcattgaacagacctcacgcggtgagcgctcttttgatat
ctattctcgtctacttaaggaacgcgtcatttttctgactggccaggttgaagaccacatggctaacctgattgtggcg
cagatgctgttcctggaagcggaaaacccagaaaaagatatctatctgtacattaactccccaggcggggtgatcactg
ccgggatgtctatctatgacaccatgcagtttatcaagcctgatgtcagcaccatctgtatgggccaggcggcctcgat
gggcgctttcttgctgaccgcaggggcaaaaggtaaacgtttttgcctgccaaattcgcgcgtgatgattcaccaaccg
ttgggcggctaccagggccaggcgaccgatatcgaaattcatgcccgtgaaattctgaaagttaaagggcgcatgaatg
aacttatggcgcttcatacgggtcaatcattagaacagattgaacgtgataccgagcgcgatcgcttcctttccgcccc
tgaagcggtggaatacggtctggtcgattcgattctgacccatcgtaattgatactagagtcacacaggactactagat
gagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaa
aatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatg
gcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttc
aatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtt
ttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagca
caaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcc
aagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagactt
gggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacc
caatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgc
tgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc