LOCUS BBa_K1911006 4966 bp DNA linear 22-Nov-2024 DEFINITION pLamdaR-LacI-GFP-DAS-pLac-ClpXP-CI ACCESSION BBa_K1911006 VERSION BBa_K1911006.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..68 /label=RBS-4\Weaker RBS 64..67 /label=RBS misc_feature 58..68 /label=BBa_B0033 RBS 58..68 /label=BBa_B0033 misc_feature 1164..1202 /label=LVA CDS 75..1202 /label=lacI-LVA misc_feature 75..1202 /label=BBa_C0012 misc_feature 1203..1227 /label=Help:Barcodes CDS 75..1202 /label=BBa_C0012 misc_feature 1240..1243 /label=conserved RBS 1236..1247 /label=BBa_B0034 CDS 1254..1970 /label=BBa_K1911005 misc_feature 1979..2017 /label=AANDENYNYADAS SsrA deg. tag misc_feature 1979..2017 /label=BBa_M0051 stem_loop 2037..2080 /label=stem_loop misc_feature 2026..2105 /label=BBa_B0010 terminator 2026..2105 /label=BBa_B0010 stem_loop 2121..2140 /label=T7 TE misc_feature 2147..2147 /label=stop polyA_site 2141..2154 /label=polya misc_feature 2114..2154 /label=BBa_B0012 terminator 2114..2154 /label=BBa_B0012 misc_feature 2165..2181 /label=lac O1 promoter 2182..2187 /label=-35 misc_feature 2188..2204 /label=lac O1 promoter 2205..2210 /label=-10 misc_feature 2163..2216 /label=BBa_R0011 promoter 2163..2216 /label=BBa_R0011 RBS 2226..2236 /label=RBS-4\Weaker RBS 2232..2235 /label=RBS misc_feature 2226..2236 /label=BBa_B0033 RBS 2226..2236 /label=BBa_B0033 CDS 2243..3517 /label=BBa_K1911003 RBS 3526..3536 /label=RBS-4\Weaker RBS 3532..3535 /label=RBS misc_feature 3526..3536 /label=BBa_B0033 RBS 3526..3536 /label=BBa_B0033 CDS 3543..4166 /label=BBa_K1911002 RBS 4175..4185 /label=RBS-4\Weaker RBS 4181..4184 /label=RBS misc_feature 4175..4185 /label=BBa_B0033 RBS 4175..4185 /label=BBa_B0033 CDS 4195..4902 /label=cI lambda misc_feature 4903..4935 /label=LVA misc_feature 4942..4966 /label=Help:Barcodes CDS 4192..4966 /label=BBa_C0051 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggacta ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 121 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 181 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 241 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 301 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 361 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 421 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 481 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 541 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 601 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 661 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 721 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 781 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 841 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 901 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 961 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 1021 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 1081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1141 tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat 1201 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggcta 1261 gcaaaggaga agaactcttc actggagttg tcccaattct tgttgaatta gatggtgatg 1321 ttaacggcca caagttctct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1381 ttaccctgaa gttcatctgc actactggca aactgcctgt tccatggcca accctggtca 1441 ctactctgtg ctatggtgtt caatgctttt caagataccc ggatcatatg aaacggcatg 1501 actttttcaa gagtgccatg cccgaaggtt atgtacagga aaggaccatc ttcttcaaag 1561 atgacggcaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 1621 gaatcgagtt aaaaggtatt gacttcaagg aagatggcaa cattctggga cacaaattgg 1681 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1741 aagtgaactt caagacccgc cacaacattg aagatggaag cgttcaacta gcagaccatt 1801 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 1861 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 1921 agtttgtaac agctgctggg attacacatg gcatggatga actgtacaat tactagaggc 1981 tgctaacgac gaaaactaca actacgctga cgcttcttac tagagccagg catcaaataa 2041 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2101 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2161 agaattgtga gcggataaca attgacattg tgagcggata acaagatact gagcacatac 2221 tagagtcaca caggactact agatgacaga taaacgcaaa gatggctcag gcaaattgct 2281 gtattgctct ttttgcggca aaagccagca tgaagtgcgc aagctgattg ccggtccatc 2341 cgtgtatatc tgcgacgaat gtgttgattt atgtaacgac atcattcgcg aagagattaa 2401 agaagttgca ccgcatcgtg aacgcagtgc gctaccgacg ccgcatgaaa ttcgcaacca 2461 cctggacgat tacgttatcg gccaggaaca ggcgaaaaaa gtgctggcgg tcgcggtata 2521 caaccattac aaacgtctgc gcaacggcga taccagcaat ggcgtcgagt tgggcaaaag 2581 taacattctg ctgatcggtc cgaccggttc cggtaaaacg ctgctggctg aaacgctggc 2641 gcgcctgctg gatgttccgt tcaccatggc cgacgcgact acactgaccg aagccggtta 2701 tgtgggtgaa gacgttgaaa acatcattca gaagctgttg cagaaatgcg actacgatgt 2761 ccagaaagca cagcgtggta ttgtctacat cgatgaaatc gacaagattt ctcgtaagtc 2821 agacaacccg tccattaccc gagacgtttc cggtgaaggc gtacagcagg cactgttgaa 2881 actgatcgaa ggtacggtag ctgctgttcc accgcaaggt gggcgtaaac atccgcagca 2941 ggagttcttg caggttgata cctctaagat cctgtttatt tgtggcggtg cgtttgccgg 3001 tctggataaa gtgatttccc accgtgtaga aaccggctcc ggcattggtt ttggcgcgac 3061 ggtaaaagcg aagtccgaca aagcaagcga aggcgagctg ctggcgcagg ttgaaccgga 3121 agatctgatc aagtttggtc ttatccctga gtttattggt cgtctgccgg ttgtcgcaac 3181 gttgaatgaa ctgagcgaag aagctctgat tcagatcctc aaagagccga aaaacgccct 3241 gaccaagcag tatcaggcgc tgtttaatct ggaaggcgtg gatctggagt tccgtgacga 3301 ggcgctggat gctatcgcta agaaagcgat ggcgcgtaaa accggtgccc gtggcctgcg 3361 ttccatcgta gaagccgcac tgctcgatac catgtacgat ctgccgtcca tggaagacgt 3421 cgaaaaagtg gttatcgacg agtcggtaat tgatggtcaa agcaaaccgt tgctgattta 3481 tggcaagccg gaagcgcaac aggcatctgg tgaataatac tagagtcaca caggactact 3541 agatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg gtgccgatgg 3601 tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt ctacttaagg 3661 aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg attgtggcgc 3721 agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac attaactccc 3781 caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt atcaagcctg 3841 atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg ctgaccgcag 3901 gggcaaaagg taaacgtttt tgcctgccaa attcgcgcgt gatgattcac caaccgttgg 3961 gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt ctgaaagtta 4021 aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa cagattgaac 4081 gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac ggtctggtcg 4141 attcgattct gacccatcgt aattgatact agagtcacac aggactacta gatgagcaca 4201 aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat 4261 gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg 4321 gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc 4381 gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga 4441 gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag 4501 taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc 4561 aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg 4621 cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac 4681 ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc 4741 agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt 4801 ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg 4861 gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac 4921 tacgctttag tagcttaata acgctgatag tgctagtgta gatcgc //