LOCUS BBa_K1911007 4919 bp DNA linear 16-Jun-2024 DEFINITION pLamdaR-LacI-GFP-pLac-ClpXP-CI ACCESSION BBa_K1911007 VERSION BBa_K1911007.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..68 /label=RBS-4\Weaker RBS 64..67 /label=RBS misc_feature 58..68 /label=BBa_B0033 RBS 58..68 /label=BBa_B0033 misc_feature 1164..1202 /label=LVA CDS 75..1202 /label=lacI-LVA misc_feature 75..1202 /label=BBa_C0012 misc_feature 1203..1227 /label=Help:Barcodes CDS 75..1202 /label=BBa_C0012 misc_feature 1240..1243 /label=conserved RBS 1236..1247 /label=BBa_B0034 CDS 1254..1970 /label=BBa_K1911005 stem_loop 1990..2033 /label=stem_loop misc_feature 1979..2058 /label=BBa_B0010 terminator 1979..2058 /label=BBa_B0010 stem_loop 2074..2093 /label=T7 TE misc_feature 2100..2100 /label=stop polyA_site 2094..2107 /label=polya misc_feature 2067..2107 /label=BBa_B0012 terminator 2067..2107 /label=BBa_B0012 misc_feature 2118..2134 /label=lac O1 promoter 2135..2140 /label=-35 misc_feature 2141..2157 /label=lac O1 promoter 2158..2163 /label=-10 misc_feature 2116..2169 /label=BBa_R0011 promoter 2116..2169 /label=BBa_R0011 RBS 2179..2189 /label=RBS-4\Weaker RBS 2185..2188 /label=RBS misc_feature 2179..2189 /label=BBa_B0033 RBS 2179..2189 /label=BBa_B0033 CDS 2196..3470 /label=BBa_K1911003 RBS 3479..3489 /label=RBS-4\Weaker RBS 3485..3488 /label=RBS misc_feature 3479..3489 /label=BBa_B0033 RBS 3479..3489 /label=BBa_B0033 CDS 3496..4119 /label=BBa_K1911002 RBS 4128..4138 /label=RBS-4\Weaker RBS 4134..4137 /label=RBS misc_feature 4128..4138 /label=BBa_B0033 RBS 4128..4138 /label=BBa_B0033 CDS 4148..4855 /label=cI lambda misc_feature 4856..4888 /label=LVA misc_feature 4895..4919 /label=Help:Barcodes CDS 4145..4919 /label=BBa_C0051 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggacta ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 121 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 181 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 241 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 301 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 361 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 421 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 481 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 541 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 601 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 661 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 721 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 781 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 841 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 901 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 961 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 1021 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 1081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1141 tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat 1201 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggcta 1261 gcaaaggaga agaactcttc actggagttg tcccaattct tgttgaatta gatggtgatg 1321 ttaacggcca caagttctct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1381 ttaccctgaa gttcatctgc actactggca aactgcctgt tccatggcca accctggtca 1441 ctactctgtg ctatggtgtt caatgctttt caagataccc ggatcatatg aaacggcatg 1501 actttttcaa gagtgccatg cccgaaggtt atgtacagga aaggaccatc ttcttcaaag 1561 atgacggcaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 1621 gaatcgagtt aaaaggtatt gacttcaagg aagatggcaa cattctggga cacaaattgg 1681 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1741 aagtgaactt caagacccgc cacaacattg aagatggaag cgttcaacta gcagaccatt 1801 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 1861 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 1921 agtttgtaac agctgctggg attacacatg gcatggatga actgtacaat tactagagcc 1981 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2041 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2101 gtttatatac tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat 2161 actgagcaca tactagagtc acacaggact actagatgac agataaacgc aaagatggct 2221 caggcaaatt gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg cgcaagctga 2281 ttgccggtcc atccgtgtat atctgcgacg aatgtgttga tttatgtaac gacatcattc 2341 gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg acgccgcatg 2401 aaattcgcaa ccacctggac gattacgtta tcggccagga acaggcgaaa aaagtgctgg 2461 cggtcgcggt atacaaccat tacaaacgtc tgcgcaacgg cgataccagc aatggcgtcg 2521 agttgggcaa aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa acgctgctgg 2581 ctgaaacgct ggcgcgcctg ctggatgttc cgttcaccat ggccgacgcg actacactga 2641 ccgaagccgg ttatgtgggt gaagacgttg aaaacatcat tcagaagctg ttgcagaaat 2701 gcgactacga tgtccagaaa gcacagcgtg gtattgtcta catcgatgaa atcgacaaga 2761 tttctcgtaa gtcagacaac ccgtccatta cccgagacgt ttccggtgaa ggcgtacagc 2821 aggcactgtt gaaactgatc gaaggtacgg tagctgctgt tccaccgcaa ggtgggcgta 2881 aacatccgca gcaggagttc ttgcaggttg atacctctaa gatcctgttt atttgtggcg 2941 gtgcgtttgc cggtctggat aaagtgattt cccaccgtgt agaaaccggc tccggcattg 3001 gttttggcgc gacggtaaaa gcgaagtccg acaaagcaag cgaaggcgag ctgctggcgc 3061 aggttgaacc ggaagatctg atcaagtttg gtcttatccc tgagtttatt ggtcgtctgc 3121 cggttgtcgc aacgttgaat gaactgagcg aagaagctct gattcagatc ctcaaagagc 3181 cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa tctggaaggc gtggatctgg 3241 agttccgtga cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt aaaaccggtg 3301 cccgtggcct gcgttccatc gtagaagccg cactgctcga taccatgtac gatctgccgt 3361 ccatggaaga cgtcgaaaaa gtggttatcg acgagtcggt aattgatggt caaagcaaac 3421 cgttgctgat ttatggcaag ccggaagcgc aacaggcatc tggtgaataa tactagagtc 3481 acacaggact actagatgtc atacagcggc gaacgagata actttgcacc ccatatggcg 3541 ctggtgccga tggtcattga acagacctca cgcggtgagc gctcttttga tatctattct 3601 cgtctactta aggaacgcgt catttttctg actggccagg ttgaagacca catggctaac 3661 ctgattgtgg cgcagatgct gttcctggaa gcggaaaacc cagaaaaaga tatctatctg 3721 tacattaact ccccaggcgg ggtgatcact gccgggatgt ctatctatga caccatgcag 3781 tttatcaagc ctgatgtcag caccatctgt atgggccagg cggcctcgat gggcgctttc 3841 ttgctgaccg caggggcaaa aggtaaacgt ttttgcctgc caaattcgcg cgtgatgatt 3901 caccaaccgt tgggcggcta ccagggccag gcgaccgata tcgaaattca tgcccgtgaa 3961 attctgaaag ttaaagggcg catgaatgaa cttatggcgc ttcatacggg tcaatcatta 4021 gaacagattg aacgtgatac cgagcgcgat cgcttccttt ccgcccctga agcggtggaa 4081 tacggtctgg tcgattcgat tctgacccat cgtaattgat actagagtca cacaggacta 4141 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct 4201 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga 4261 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa 4321 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc 4381 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag 4441 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct 4501 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga 4561 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc 4621 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga 4681 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag 4741 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag 4801 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc 4861 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgc //