##gff-version 3
##sequence-region BBa_K1911007 1 4919
BBa_K1911007	.	ribosome_entry_site	2179	2189	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	2179	2189	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911007	.	engineered_region	2179	2189	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	2185	2188	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	3479	3489	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	3479	3489	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911007	.	engineered_region	3479	3489	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	3485	3488	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911007	.	CDS	4145	4919	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K1911007	.	engineered_tag	4895	4919	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1911007	.	CDS	4148	4855	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1911007	.	tag	4856	4888	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1911007	.	CDS	2196	3470	.	+	0	ID=BBa_K1911003;Name=BBa_K1911003
BBa_K1911007	.	ribosome_entry_site	58	68	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	58	68	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911007	.	engineered_region	58	68	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911007	.	CDS	3496	4119	.	+	0	ID=BBa_K1911002;Name=BBa_K1911002
BBa_K1911007	.	ribosome_entry_site	4128	4138	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	4128	4138	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1911007	.	engineered_region	4128	4138	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	4134	4137	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1911007	.	ribosome_entry_site	1236	1247	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1911007	.	conserved_region	1240	1243	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1911007	.	CDS	75	1202	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1911007	.	engineered_tag	1203	1227	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1911007	.	CDS	75	1202	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1911007	.	engineered_region	75	1202	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1911007	.	tag	1164	1202	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1911007	.	CDS	1254	1970	.	+	0	ID=BBa_K1911005;Name=BBa_K1911005
BBa_K1911007	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1911007	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1911007	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1911007	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1911007	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1911007	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1911007	.	promoter	2116	2169	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1911007	.	operator	2141	2157	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1911007	.	engineered_region	2116	2169	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1911007	.	promoter	2135	2140	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1911007	.	operator	2118	2134	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1911007	.	promoter	2158	2163	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1911007	.	terminator	1979	2058	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1911007	.	stem_loop	1990	2033	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1911007	.	engineered_region	1979	2058	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1911007	.	terminator	2067	2107	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1911007	.	stem_loop	2074	2093	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1911007	.	stop_codon	2100	2100	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1911007	.	polyA_site	2094	2107	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1911007	.	engineered_region	2067	2107	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1911007
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggactactagatggt
gaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaac
caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcg
tggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgca
aattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtc
gaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgacc
aggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaa
cagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcg
ctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa
ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgaggg
catcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctg
cgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacca
tcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaaggg
caatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcg
ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacg
ctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggctagcaa
aggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttc
catggccaaccctggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggca
acattctgggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat
caaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca
attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa
agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaattacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataaca
attgacattgtgagcggataacaagatactgagcacatactagagtcacacaggactactagatgacagataaacgcaa
agatggctcaggcaaattgctgtattgctctttttgcggcaaaagccagcatgaagtgcgcaagctgattgccggtcca
tccgtgtatatctgcgacgaatgtgttgatttatgtaacgacatcattcgcgaagagattaaagaagttgcaccgcatc
gtgaacgcagtgcgctaccgacgccgcatgaaattcgcaaccacctggacgattacgttatcggccaggaacaggcgaa
aaaagtgctggcggtcgcggtatacaaccattacaaacgtctgcgcaacggcgataccagcaatggcgtcgagttgggc
aaaagtaacattctgctgatcggtccgaccggttccggtaaaacgctgctggctgaaacgctggcgcgcctgctggatg
ttccgttcaccatggccgacgcgactacactgaccgaagccggttatgtgggtgaagacgttgaaaacatcattcagaa
gctgttgcagaaatgcgactacgatgtccagaaagcacagcgtggtattgtctacatcgatgaaatcgacaagatttct
cgtaagtcagacaacccgtccattacccgagacgtttccggtgaaggcgtacagcaggcactgttgaaactgatcgaag
gtacggtagctgctgttccaccgcaaggtgggcgtaaacatccgcagcaggagttcttgcaggttgatacctctaagat
cctgtttatttgtggcggtgcgtttgccggtctggataaagtgatttcccaccgtgtagaaaccggctccggcattggt
tttggcgcgacggtaaaagcgaagtccgacaaagcaagcgaaggcgagctgctggcgcaggttgaaccggaagatctga
tcaagtttggtcttatccctgagtttattggtcgtctgccggttgtcgcaacgttgaatgaactgagcgaagaagctct
gattcagatcctcaaagagccgaaaaacgccctgaccaagcagtatcaggcgctgtttaatctggaaggcgtggatctg
gagttccgtgacgaggcgctggatgctatcgctaagaaagcgatggcgcgtaaaaccggtgcccgtggcctgcgttcca
tcgtagaagccgcactgctcgataccatgtacgatctgccgtccatggaagacgtcgaaaaagtggttatcgacgagtc
ggtaattgatggtcaaagcaaaccgttgctgatttatggcaagccggaagcgcaacaggcatctggtgaataatactag
agtcacacaggactactagatgtcatacagcggcgaacgagataactttgcaccccatatggcgctggtgccgatggtc
attgaacagacctcacgcggtgagcgctcttttgatatctattctcgtctacttaaggaacgcgtcatttttctgactg
gccaggttgaagaccacatggctaacctgattgtggcgcagatgctgttcctggaagcggaaaacccagaaaaagatat
ctatctgtacattaactccccaggcggggtgatcactgccgggatgtctatctatgacaccatgcagtttatcaagcct
gatgtcagcaccatctgtatgggccaggcggcctcgatgggcgctttcttgctgaccgcaggggcaaaaggtaaacgtt
tttgcctgccaaattcgcgcgtgatgattcaccaaccgttgggcggctaccagggccaggcgaccgatatcgaaattca
tgcccgtgaaattctgaaagttaaagggcgcatgaatgaacttatggcgcttcatacgggtcaatcattagaacagatt
gaacgtgataccgagcgcgatcgcttcctttccgcccctgaagcggtggaatacggtctggtcgattcgattctgaccc
atcgtaattgatactagagtcacacaggactactagatgagcacaaaaaagaaaccattaacacaagagcagcttgagg
acgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagat
ggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgca
aaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagta
tgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttag
aacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggtt
gaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagc
aggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatag
cggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaa
gttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgct
gatagtgctagtgtagatcgc