BBa_K1913000 1 BBa_K1913000 chiA for Varroa destructor 2016-09-21T11:00:00Z 2016-09-22T06:18:00Z The source of this part is chiA from Serratia marcescens GEI; accession GQ855217. Serratia marcescens GEI chiA protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiA has both exochitinase and endochitinase activity. This protein is known to show synergistic activity with ChiB. ChiA can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiA. false false _2380_ 29556 29556 9 false This sequence was optimized using the IDT codon optimization tool. Multiple restriction sites were removed; BsaI at position 195, PstI at position 644, PstI at position 905 and EcoRI at position 943. Additionally, several antisense stop codons were introduced to prevent antisense transcription. false Lisa R??ttjers annotation2484442 1 TAA stop codon range2484442 1 1690 1692 annotation2484444 1 chiA coding region range2484444 1 1 1695 annotation2484443 1 TAA stop codon range2484443 1 1693 1695 annotation2484441 1 ATG start codon range2484441 1 1 3 BBa_K1913000_sequence 1 atgcgcaagttcaataaaccccttctggctttgctgatcggctcaaccctttgctcagccgcgcaagcggctgcaccggggaaaccaactattgcttgggggaatactaaattcgctatcgttgaagtggaccaggcagccacggcttataataatcttgttaaggtaaaaaatgcggcagacgtctcggtaagttggaatttgtggaatggggatgccggtacaacagcaaaaatcctgttgaacggtaaggaagcctggagcggcccgtcaactggatcgtcaggaaccgcgaacttcaaggtcaacaagggcggacgttatcaaatgcaggtcgctctttgcaacgccgacggatgcacggcaagtgacgccaccgaaatcgtagttgctgatacggacggctcccacttggccccccttaaagagcctttacttgagaagaacaaaccttataagcaaaattttggcaaagttgtaggtagttattttgtagagtggggcgtatatggacgtaacttcactgtggataaaattccagcccaaaatttgacccacttgttgtatggatttattcctatttgcggcgggaacggaatcaatgactccctgaaggagattgaaggctcatttcaagctttgcagcgttcctgtcagggccgcgaggactttaaagtctcaatccacgatcctttcgcagctatgcaaaaggcccagaaaggagtcacggcttgggatgatccatataagggcaactttggtcagctgatggctctgaaacaggcgcgtcccgatttgaaaattttgcccagcattggaggatggacgctgtccgacccgttcttctttatgggcgataaggttaaacgcgaccgttttgtaggttcagtaaaggagttcctgcaaacctggaagttctttgatggggtagatattgattgggagtttcctggtgggaagggcgctaatccaaacctgggctctcctcaggacggagaaacctatgttcttttgatgaaagagctgcgcgccatgttggaccaattatccgtggaaactgggcgcaaatatgaactgacttctgctatctctgccggtaaggacaagattgataaggtcgcatataacgttgcccagaactcgatggatcacgtatttcttatgtcttacgatttttatggcgcattcgatttgaagaatcttgggcaccagactgcccttaacgcccctgcttggaaacccgataccgcctacactacggttaatggcgtaaatgcgctgttggctcagggcgtcactccaggaaaaatcgttgtaggaacggcaatgtatggccgtggttggacaggtgtcaatgggtaccagaacaatatcccgtttacgggcacggctacgggcccggttaaaggcacgtgggagaacgggatcgtagactaccgccaaatcgctagtcaatttatgtccggcgaatggcagtatacttatgatgccactgcggaggcaccctatgtcttcaagccgtctactggggatctgatcaccttcgacgatgcacgctccgtccaggccaaggggaaatacgtgcttgacaaacagttaggcggattgtttagctgggaaattgacgcggataacggcgatattcttaactctatgaatgcctcgttaggaaattccgcaggggtgcaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z