BBa_K1913001 1 BBa_K1913001 chiB for Varroa destructor 2016-09-21T11:00:00Z 2016-09-22T06:27:24Z This part is based on chiB from Serratia marcescens GEI; accession GQ855218. Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB. false false _2380_ 29556 29556 9 false This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription. false Lisa R??ttjers annotation2484447 1 TAA stop codon range2484447 1 1501 1503 annotation2484446 1 TAA stop codon range2484446 1 1498 1500 annotation2484445 1 ATG start codon range2484445 1 1 3 annotation2484448 1 chiB coding region range2484448 1 1 1503 BBa_K1913001_sequence 1 atgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagctgacccgcgccttccccagcccgttcagcctgacggtcgatgccgcggtgcagcaacacctgatgatggaaggggtgccgagcgccaaaatcgtgatgggcgtgccgttttacggccgcgccttcaagggcgtcagcggcggcaacggcgggcaatacagcagccacagcacgccgggcgaagatccgtatccgagcaccgactactggctggtgggctgcgaagagtgtgtgcgcgacaaggatccgcgcatcgcgtcctatcgccagctggagcagatgcttcagggcaactacggctatcagcggttgtggaacgacaagaccaaaaccccttacctgtatcacgcgcagaacgggctgttcgtcacctatgacgacgccgagagcttcaaatacaaagcgaagtacatcaagcagcagcagctgggcggcgtgatgttctggcacctggggcaagacaaccgcaacggcgatctgctggccgcgctggatcgctatttcaacgccgcggactacgacgacagccagctggatatgggcaccggcctgcgctacaccggcgtcggccccggcaacctgccgatcatgacggcaccggcctacgtgccgggcaccatttacgcgcagggcgcgctggtgtcctatcagggttacgtctggcagaccaagtgggggtacatcacctctgcgccgggttcagacagcgcctggctgaaggtgggccgcgtagcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z