BBa_K1913001
1
BBa_K1913001
chiB for Varroa destructor
2016-09-21T11:00:00Z
2016-09-22T06:27:24Z
This part is based on chiB from Serratia marcescens GEI; accession GQ855218.
Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB.
false
false
_2380_
29556
29556
9
false
This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription.
false
Lisa R??ttjers
annotation2484447
1
TAA stop codon
range2484447
1
1501
1503
annotation2484446
1
TAA stop codon
range2484446
1
1498
1500
annotation2484445
1
ATG start codon
range2484445
1
1
3
annotation2484448
1
chiB coding region
range2484448
1
1
1503
BBa_K1913001_sequence
1
atgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagctgacccgcgccttccccagcccgttcagcctgacggtcgatgccgcggtgcagcaacacctgatgatggaaggggtgccgagcgccaaaatcgtgatgggcgtgccgttttacggccgcgccttcaagggcgtcagcggcggcaacggcgggcaatacagcagccacagcacgccgggcgaagatccgtatccgagcaccgactactggctggtgggctgcgaagagtgtgtgcgcgacaaggatccgcgcatcgcgtcctatcgccagctggagcagatgcttcagggcaactacggctatcagcggttgtggaacgacaagaccaaaaccccttacctgtatcacgcgcagaacgggctgttcgtcacctatgacgacgccgagagcttcaaatacaaagcgaagtacatcaagcagcagcagctgggcggcgtgatgttctggcacctggggcaagacaaccgcaacggcgatctgctggccgcgctggatcgctatttcaacgccgcggactacgacgacagccagctggatatgggcaccggcctgcgctacaccggcgtcggccccggcaacctgccgatcatgacggcaccggcctacgtgccgggcaccatttacgcgcagggcgcgctggtgtcctatcagggttacgtctggcagaccaagtgggggtacatcacctctgcgccgggttcagacagcgcctggctgaaggtgggccgcgtagcgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z