LOCUS       BBa_K1913002            3069 bp    DNA     linear       16-Jun-2024
DEFINITION  chiA device regulated by pBAD
ACCESSION   BBa_K1913002
VERSION     BBa_K1913002.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1231
                     /label=RBS-3\Weak
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1231
                     /label=BBa_B0032
     RBS             1219..1231
                     /label=BBa_B0032
     misc_feature    1238..1240
                     /label=ATG start codon
     misc_feature    2927..2929
                     /label=TAA stop codon
     misc_feature    2930..2932
                     /label=TAA stop codon
     CDS             1238..2932
                     /label=chiA coding region
     CDS             1238..2932
                     /label=BBa_K1913000
     stem_loop       2952..2995
                     /label=stem_loop
     misc_feature    2941..3020
                     /label=BBa_B0010
     terminator      2941..3020
                     /label=BBa_B0010
     stem_loop       3036..3055
                     /label=T7 TE
     misc_feature    3062..3062
                     /label=stop
     polyA_site      3056..3069
                     /label=polya
     misc_feature    3029..3069
                     /label=BBa_B0012
     terminator      3029..3069
                     /label=BBa_B0012
     terminator      2941..3069
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg cgcaagttca ataaacccct
     1261 tctggctttg ctgatcggct caaccctttg ctcagccgcg caagcggctg caccggggaa
     1321 accaactatt gcttggggga atactaaatt cgctatcgtt gaagtggacc aggcagccac
     1381 ggcttataat aatcttgtta aggtaaaaaa tgcggcagac gtctcggtaa gttggaattt
     1441 gtggaatggg gatgccggta caacagcaaa aatcctgttg aacggtaagg aagcctggag
     1501 cggcccgtca actggatcgt caggaaccgc gaacttcaag gtcaacaagg gcggacgtta
     1561 tcaaatgcag gtcgctcttt gcaacgccga cggatgcacg gcaagtgacg ccaccgaaat
     1621 cgtagttgct gatacggacg gctcccactt ggcccccctt aaagagcctt tacttgagaa
     1681 gaacaaacct tataagcaaa attttggcaa agttgtaggt agttattttg tagagtgggg
     1741 cgtatatgga cgtaacttca ctgtggataa aattccagcc caaaatttga cccacttgtt
     1801 gtatggattt attcctattt gcggcgggaa cggaatcaat gactccctga aggagattga
     1861 aggctcattt caagctttgc agcgttcctg tcagggccgc gaggacttta aagtctcaat
     1921 ccacgatcct ttcgcagcta tgcaaaaggc ccagaaagga gtcacggctt gggatgatcc
     1981 atataagggc aactttggtc agctgatggc tctgaaacag gcgcgtcccg atttgaaaat
     2041 tttgcccagc attggaggat ggacgctgtc cgacccgttc ttctttatgg gcgataaggt
     2101 taaacgcgac cgttttgtag gttcagtaaa ggagttcctg caaacctgga agttctttga
     2161 tggggtagat attgattggg agtttcctgg tgggaagggc gctaatccaa acctgggctc
     2221 tcctcaggac ggagaaacct atgttctttt gatgaaagag ctgcgcgcca tgttggacca
     2281 attatccgtg gaaactgggc gcaaatatga actgacttct gctatctctg ccggtaagga
     2341 caagattgat aaggtcgcat ataacgttgc ccagaactcg atggatcacg tatttcttat
     2401 gtcttacgat ttttatggcg cattcgattt gaagaatctt gggcaccaga ctgcccttaa
     2461 cgcccctgct tggaaacccg ataccgccta cactacggtt aatggcgtaa atgcgctgtt
     2521 ggctcagggc gtcactccag gaaaaatcgt tgtaggaacg gcaatgtatg gccgtggttg
     2581 gacaggtgtc aatgggtacc agaacaatat cccgtttacg ggcacggcta cgggcccggt
     2641 taaaggcacg tgggagaacg ggatcgtaga ctaccgccaa atcgctagtc aatttatgtc
     2701 cggcgaatgg cagtatactt atgatgccac tgcggaggca ccctatgtct tcaagccgtc
     2761 tactggggat ctgatcacct tcgacgatgc acgctccgtc caggccaagg ggaaatacgt
     2821 gcttgacaaa cagttaggcg gattgtttag ctgggaaatt gacgcggata acggcgatat
     2881 tcttaactct atgaatgcct cgttaggaaa ttccgcaggg gtgcaataat aatactagag
     2941 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3001 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     3061 gcgtttata
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