BBa_K1913002 1 BBa_K1913002 chiA device regulated by pBAD 2016-09-21T11:00:00Z 2016-09-29T07:17:24Z Original chiA sequence originates from GQ855217. This part uses a pBAD promoter (BBa_I0500), weak RBS (BBa_B0032) and a double terminator (BBa_B0015). When induced with arabinose, it will produce chiA. A weak promoter was chosen to prevent formation of inclusion bodies during expression. false false _2380_ 29556 29556 9 false This is a composite part for expression of the chiA protein coding region from BBa_K1913000. false Lisa R??ttjers component2484460 1 BBa_K1913000 component2484454 1 BBa_B0032 component2484467 1 BBa_B0015 component2484452 1 BBa_I0500 annotation2484452 1 BBa_I0500 range2484452 1 1 1210 annotation2484454 1 BBa_B0032 range2484454 1 1219 1231 annotation2484460 1 BBa_K1913000 range2484460 1 1238 2932 annotation2484467 1 BBa_B0015 range2484467 1 2941 3069 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1913000 1 BBa_K1913000 chiA for Varroa destructor 2016-09-21T11:00:00Z 2016-09-22T06:18:00Z The source of this part is chiA from Serratia marcescens GEI; accession GQ855217. Serratia marcescens GEI chiA protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiA has both exochitinase and endochitinase activity. This protein is known to show synergistic activity with ChiB. ChiA can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiA. false false _2380_ 29556 29556 9 false This sequence was optimized using the IDT codon optimization tool. Multiple restriction sites were removed; BsaI at position 195, PstI at position 644, PstI at position 905 and EcoRI at position 943. Additionally, several antisense stop codons were introduced to prevent antisense transcription. false Lisa R??ttjers annotation2484444 1 chiA coding region range2484444 1 1 1695 annotation2484441 1 ATG start codon range2484441 1 1 3 annotation2484442 1 TAA stop codon range2484442 1 1690 1692 annotation2484443 1 TAA stop codon range2484443 1 1693 1695 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1913002_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatgcgcaagttcaataaaccccttctggctttgctgatcggctcaaccctttgctcagccgcgcaagcggctgcaccggggaaaccaactattgcttgggggaatactaaattcgctatcgttgaagtggaccaggcagccacggcttataataatcttgttaaggtaaaaaatgcggcagacgtctcggtaagttggaatttgtggaatggggatgccggtacaacagcaaaaatcctgttgaacggtaaggaagcctggagcggcccgtcaactggatcgtcaggaaccgcgaacttcaaggtcaacaagggcggacgttatcaaatgcaggtcgctctttgcaacgccgacggatgcacggcaagtgacgccaccgaaatcgtagttgctgatacggacggctcccacttggccccccttaaagagcctttacttgagaagaacaaaccttataagcaaaattttggcaaagttgtaggtagttattttgtagagtggggcgtatatggacgtaacttcactgtggataaaattccagcccaaaatttgacccacttgttgtatggatttattcctatttgcggcgggaacggaatcaatgactccctgaaggagattgaaggctcatttcaagctttgcagcgttcctgtcagggccgcgaggactttaaagtctcaatccacgatcctttcgcagctatgcaaaaggcccagaaaggagtcacggcttgggatgatccatataagggcaactttggtcagctgatggctctgaaacaggcgcgtcccgatttgaaaattttgcccagcattggaggatggacgctgtccgacccgttcttctttatgggcgataaggttaaacgcgaccgttttgtaggttcagtaaaggagttcctgcaaacctggaagttctttgatggggtagatattgattgggagtttcctggtgggaagggcgctaatccaaacctgggctctcctcaggacggagaaacctatgttcttttgatgaaagagctgcgcgccatgttggaccaattatccgtggaaactgggcgcaaatatgaactgacttctgctatctctgccggtaaggacaagattgataaggtcgcatataacgttgcccagaactcgatggatcacgtatttcttatgtcttacgatttttatggcgcattcgatttgaagaatcttgggcaccagactgcccttaacgcccctgcttggaaacccgataccgcctacactacggttaatggcgtaaatgcgctgttggctcagggcgtcactccaggaaaaatcgttgtaggaacggcaatgtatggccgtggttggacaggtgtcaatgggtaccagaacaatatcccgtttacgggcacggctacgggcccggttaaaggcacgtgggagaacgggatcgtagactaccgccaaatcgctagtcaatttatgtccggcgaatggcagtatacttatgatgccactgcggaggcaccctatgtcttcaagccgtctactggggatctgatcaccttcgacgatgcacgctccgtccaggccaaggggaaatacgtgcttgacaaacagttaggcggattgtttagctgggaaattgacgcggataacggcgatattcttaactctatgaatgcctcgttaggaaattccgcaggggtgcaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_K1913000_sequence 1 atgcgcaagttcaataaaccccttctggctttgctgatcggctcaaccctttgctcagccgcgcaagcggctgcaccggggaaaccaactattgcttgggggaatactaaattcgctatcgttgaagtggaccaggcagccacggcttataataatcttgttaaggtaaaaaatgcggcagacgtctcggtaagttggaatttgtggaatggggatgccggtacaacagcaaaaatcctgttgaacggtaaggaagcctggagcggcccgtcaactggatcgtcaggaaccgcgaacttcaaggtcaacaagggcggacgttatcaaatgcaggtcgctctttgcaacgccgacggatgcacggcaagtgacgccaccgaaatcgtagttgctgatacggacggctcccacttggccccccttaaagagcctttacttgagaagaacaaaccttataagcaaaattttggcaaagttgtaggtagttattttgtagagtggggcgtatatggacgtaacttcactgtggataaaattccagcccaaaatttgacccacttgttgtatggatttattcctatttgcggcgggaacggaatcaatgactccctgaaggagattgaaggctcatttcaagctttgcagcgttcctgtcagggccgcgaggactttaaagtctcaatccacgatcctttcgcagctatgcaaaaggcccagaaaggagtcacggcttgggatgatccatataagggcaactttggtcagctgatggctctgaaacaggcgcgtcccgatttgaaaattttgcccagcattggaggatggacgctgtccgacccgttcttctttatgggcgataaggttaaacgcgaccgttttgtaggttcagtaaaggagttcctgcaaacctggaagttctttgatggggtagatattgattgggagtttcctggtgggaagggcgctaatccaaacctgggctctcctcaggacggagaaacctatgttcttttgatgaaagagctgcgcgccatgttggaccaattatccgtggaaactgggcgcaaatatgaactgacttctgctatctctgccggtaaggacaagattgataaggtcgcatataacgttgcccagaactcgatggatcacgtatttcttatgtcttacgatttttatggcgcattcgatttgaagaatcttgggcaccagactgcccttaacgcccctgcttggaaacccgataccgcctacactacggttaatggcgtaaatgcgctgttggctcagggcgtcactccaggaaaaatcgttgtaggaacggcaatgtatggccgtggttggacaggtgtcaatgggtaccagaacaatatcccgtttacgggcacggctacgggcccggttaaaggcacgtgggagaacgggatcgtagactaccgccaaatcgctagtcaatttatgtccggcgaatggcagtatacttatgatgccactgcggaggcaccctatgtcttcaagccgtctactggggatctgatcaccttcgacgatgcacgctccgtccaggccaaggggaaatacgtgcttgacaaacagttaggcggattgtttagctgggaaattgacgcggataacggcgatattcttaactctatgaatgcctcgttaggaaattccgcaggggtgcaataataa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z