LOCUS BBa_K1913003 2877 bp DNA linear 29-Nov-2024 DEFINITION chiB device regulated by pBAD ACCESSION BBa_K1913003 VERSION BBa_K1913003.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 misc_feature 1238..1240 /label=ATG start codon misc_feature 2735..2737 /label=TAA stop codon misc_feature 2738..2740 /label=TAA stop codon CDS 1238..2740 /label=chiB coding region CDS 1238..2740 /label=BBa_K1913001 stem_loop 2760..2803 /label=stem_loop misc_feature 2749..2828 /label=BBa_B0010 terminator 2749..2828 /label=BBa_B0010 stem_loop 2844..2863 /label=T7 TE misc_feature 2870..2870 /label=stop polyA_site 2864..2877 /label=polya misc_feature 2837..2877 /label=BBa_B0012 terminator 2837..2877 /label=BBa_B0012 terminator 2749..2877 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg tccacacgta aagccgttat 1261 tgggtattat tttattccaa ccaaccaaat caataattac accgagtccg atacctccat 1321 cgtgccattc ccggtatcca acatcacgcc ggccaaagcc aaacagctga cgcacatcaa 1381 cttctctttc ctggatatca acagcaacct ggagtgcgcc tgggatccgg ccaccaacga 1441 cgccaaagcg cgcgatgtgg tcaagcgtct gaccgcgctc aaagcgcaca accccaacct 1501 gcgcatcatg ttctccatcg gcggctggta ctactccaac gatctgggcg tgtcgcacgc 1561 caactacgtc aacgcggtga aaaccccggc gtcgcgcacc aagttcgccc aatcctgcgt 1621 acgcatcatg aaggattacg gcttcgacgg cgtggacatc gactgggagt atccgcaagc 1681 ggcagaagtg gacggcttca tcgccgcgct gcaagagatc cgcaccctgc tgaatcagca 1741 aaccgttgct gacggccgcc aagcactgcc gtatcagttg accatcgccg gcgccggcgg 1801 cgctttcttc ctgtcgcgct attacagcaa gctggcgcag atcgtcgcgc cgctcgatta 1861 catcaacctg atgacctacg atctggccgg cccctgggag aagatcacca accaccaggc 1921 ggcgctgttc ggcgacgcgg ccgggccgac cttctacaat gcgctgcgcg aagccaatct 1981 gggctggagc tgggaagagc tgacccgcgc cttccccagc ccgttcagcc tgacggtcga 2041 tgccgcggtg cagcaacacc tgatgatgga aggggtgccg agcgccaaaa tcgtgatggg 2101 cgtgccgttt tacggccgcg ccttcaaggg cgtcagcggc ggcaacggcg ggcaatacag 2161 cagccacagc acgccgggcg aagatccgta tccgagcacc gactactggc tggtgggctg 2221 cgaagagtgt gtgcgcgaca aggatccgcg catcgcgtcc tatcgccagc tggagcagat 2281 gcttcagggc aactacggct atcagcggtt gtggaacgac aagaccaaaa ccccttacct 2341 gtatcacgcg cagaacgggc tgttcgtcac ctatgacgac gccgagagct tcaaatacaa 2401 agcgaagtac atcaagcagc agcagctggg cggcgtgatg ttctggcacc tggggcaaga 2461 caaccgcaac ggcgatctgc tggccgcgct ggatcgctat ttcaacgccg cggactacga 2521 cgacagccag ctggatatgg gcaccggcct gcgctacacc ggcgtcggcc ccggcaacct 2581 gccgatcatg acggcaccgg cctacgtgcc gggcaccatt tacgcgcagg gcgcgctggt 2641 gtcctatcag ggttacgtct ggcagaccaa gtgggggtac atcacctctg cgccgggttc 2701 agacagcgcc tggctgaagg tgggccgcgt agcgtaataa tactagagcc aggcatcaaa 2761 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2821 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //