BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498842 1 PC range1498842 1 1043 1114 annotation1498843 1 AraC range1498843 1 1 1079 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1913003 1 BBa_K1913003 chiB device regulated by pBAD 2016-09-21T11:00:00Z 2016-09-29T07:18:40Z Original chiB sequence originates from GQ855218. This part uses a pBAD promoter (BBa_I0500), weak RBS (BBa_B0032), chiB protein coding sequence (BBa_K1913001) and a double terminator (BBa_B0015). When induced with arabinose, it will produce chiB. A weak promoter was chosen to prevent formation of inclusion bodies during expression. false false _2380_ 29556 29556 9 false This is a composite part for expression of the chiB protein coding region from BBa_K1913001. false Lisa R??ttjers component2484471 1 BBa_I0500 component2484479 1 BBa_K1913001 component2484486 1 BBa_B0015 component2484473 1 BBa_B0032 annotation2484471 1 BBa_I0500 range2484471 1 1 1210 annotation2484473 1 BBa_B0032 range2484473 1 1219 1231 annotation2484486 1 BBa_B0015 range2484486 1 2749 2877 annotation2484479 1 BBa_K1913001 range2484479 1 1238 2740 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1913001 1 BBa_K1913001 chiB for Varroa destructor 2016-09-21T11:00:00Z 2016-09-22T06:27:24Z This part is based on chiB from Serratia marcescens GEI; accession GQ855218. Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB. false false _2380_ 29556 29556 9 false This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription. false Lisa R??ttjers annotation2484447 1 TAA stop codon range2484447 1 1501 1503 annotation2484446 1 TAA stop codon range2484446 1 1498 1500 annotation2484445 1 ATG start codon range2484445 1 1 3 annotation2484448 1 chiB coding region range2484448 1 1 1503 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1913003_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagctgacccgcgccttccccagcccgttcagcctgacggtcgatgccgcggtgcagcaacacctgatgatggaaggggtgccgagcgccaaaatcgtgatgggcgtgccgttttacggccgcgccttcaagggcgtcagcggcggcaacggcgggcaatacagcagccacagcacgccgggcgaagatccgtatccgagcaccgactactggctggtgggctgcgaagagtgtgtgcgcgacaaggatccgcgcatcgcgtcctatcgccagctggagcagatgcttcagggcaactacggctatcagcggttgtggaacgacaagaccaaaaccccttacctgtatcacgcgcagaacgggctgttcgtcacctatgacgacgccgagagcttcaaatacaaagcgaagtacatcaagcagcagcagctgggcggcgtgatgttctggcacctggggcaagacaaccgcaacggcgatctgctggccgcgctggatcgctatttcaacgccgcggactacgacgacagccagctggatatgggcaccggcctgcgctacaccggcgtcggccccggcaacctgccgatcatgacggcaccggcctacgtgccgggcaccatttacgcgcagggcgcgctggtgtcctatcagggttacgtctggcagaccaagtgggggtacatcacctctgcgccgggttcagacagcgcctggctgaaggtgggccgcgtagcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1913001_sequence 1 atgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagctgacccgcgccttccccagcccgttcagcctgacggtcgatgccgcggtgcagcaacacctgatgatggaaggggtgccgagcgccaaaatcgtgatgggcgtgccgttttacggccgcgccttcaagggcgtcagcggcggcaacggcgggcaatacagcagccacagcacgccgggcgaagatccgtatccgagcaccgactactggctggtgggctgcgaagagtgtgtgcgcgacaaggatccgcgcatcgcgtcctatcgccagctggagcagatgcttcagggcaactacggctatcagcggttgtggaacgacaagaccaaaaccccttacctgtatcacgcgcagaacgggctgttcgtcacctatgacgacgccgagagcttcaaatacaaagcgaagtacatcaagcagcagcagctgggcggcgtgatgttctggcacctggggcaagacaaccgcaacggcgatctgctggccgcgctggatcgctatttcaacgccgcggactacgacgacagccagctggatatgggcaccggcctgcgctacaccggcgtcggccccggcaacctgccgatcatgacggcaccggcctacgtgccgggcaccatttacgcgcagggcgcgctggtgtcctatcagggttacgtctggcagaccaagtgggggtacatcacctctgcgccgggttcagacagcgcctggctgaaggtgggccgcgtagcgtaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z