BBa_K1913003
type: ComponentDefinition
persistent identity: BBa K1913003
display id: BBa_K1913003
version: 1
was generated by: iGEM to SBOL conversion
description: chiB device regulated by pBAD
created: 2016-09-21T11:00:00Z
modified: 2016-09-29T07:18:40Z
mutable provenance: Original chiB sequence originates from GQ855218.
owned by: james
owned by: myers
mutable description: This part uses a pBAD promoter (BBa_I0500), weak RBS (BBa_B0032), chiB protein coding sequence (BBa_K1913001) and a double terminator (BBa_B0015). When induced with arabinose, it will produce chiB. A weak promoter was chosen to prevent formation of inclusion bodies during expression.
discontinued: false
dominant: false
experience: experience/None
group u list: _2380_
m user id: 29556
owner id: 29556
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This is a composite part for expression of the chiB protein coding region from BBa_K1913001.
star: false
creator: Lisa R??ttjers
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1913001
component: BBa_B0032
component: BBa_I0500
component: BBa_B0015
sequence annotation: BBa_I0500
sequence annotation: BBa_B0032
sequence annotation: BBa_K1913001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1913003_sequence/1

BBa_K1913001
type: Component
persistent identity: component2484479
display id: component2484479
version: 1
top level: BBa_K1913003
definition: BBa_K1913001
access: public

BBa_B0032
type: Component
persistent identity: component2484473
display id: component2484473
version: 1
top level: BBa_K1913003
definition: BBa_B0032
access: public

BBa_I0500
type: Component
persistent identity: component2484471
display id: component2484471
version: 1
top level: BBa_K1913003
definition: pBad/araC
access: public

BBa_B0015
type: Component
persistent identity: component2484486
display id: component2484486
version: 1
top level: BBa_K1913003
definition: BBa_B0015
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2484471
display id: annotation2484471
version: 1
top level: BBa_K1913003
location: https://synbiohub.org/public/igem/BBa_K1913003/annotation2484471/range2484471/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1913003/annotation2484471/range2484471/1
type: Range
persistent identity: range2484471
display id: range2484471
version: 1
top level: BBa_K1913003
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2484473
display id: annotation2484473
version: 1
top level: BBa_K1913003
location: https://synbiohub.org/public/igem/BBa_K1913003/annotation2484473/range2484473/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1913003/annotation2484473/range2484473/1
type: Range
persistent identity: range2484473
display id: range2484473
version: 1
top level: BBa_K1913003
direction: direction/forward
start: 1219
end: 1231
orientation: inline

BBa_K1913001
type: SequenceAnnotation
persistent identity: annotation2484479
display id: annotation2484479
version: 1
top level: BBa_K1913003
location: https://synbiohub.org/public/igem/BBa_K1913003/annotation2484479/range2484479/1
component: BBa_K1913001

https://synbiohub.org/public/igem/BBa_K1913003/annotation2484479/range2484479/1
type: Range
persistent identity: range2484479
display id: range2484479
version: 1
top level: BBa_K1913003
direction: direction/reverse
start: 1238
end: 2740
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2484486
display id: annotation2484486
version: 1
top level: BBa_K1913003
location: https://synbiohub.org/public/igem/BBa_K1913003/annotation2484486/range2484486/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1913003/annotation2484486/range2484486/1
type: Range
persistent identity: range2484486
display id: range2484486
version: 1
top level: BBa_K1913003
direction: direction/reverse
start: 2749
end: 2877
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

BBa_K1913001
type: ComponentDefinition
persistent identity: BBa K1913001
display id: BBa_K1913001
version: 1
was generated by: iGEM to SBOL conversion
description: chiB for Varroa destructor
created: 2016-09-21T11:00:00Z
modified: 2016-09-22T06:27:24Z
mutable provenance: This part is based on chiB from Serratia marcescens GEI; accession GQ855218.
owned by: james
owned by: myers
mutable description: Serratia marcescens GEI chiB protein coding region optimized for BioBrick use. This part is useful for breaking down chitin polymers. The strain of origin has been isolated from the Chinese honey bee and showed virulence towards Varroa destructor. Chitinases A and B from the strain were found to have miticidal activity. ChiB has exochitinase activity. This protein is known to show synergistic activity with ChiA. ChiB can potentially be used to control Varroa destructor mite populations, as mite mortality increases when the mites are subjected to filter paper incubated with purified chiB.
discontinued: false
dominant: false
group u list: _2380_
m user id: 29556
owner id: 29556
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This sequence was optimized using the IDT codon optimization tool. One restriction site was removed; PstI at position 1049. Additionally, several antisense stop codons were introduced to prevent antisense transcription.
star: false
creator: Lisa R??ttjers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG start codon
sequence annotation: chiB coding region
sequence annotation: TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1913001_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2484445
display id: annotation2484445
version: 1
top level: BBa_K1913001
location: https://synbiohub.org/public/igem/BBa_K1913001/annotation2484445/range2484445/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1913001/annotation2484445/range2484445/1
type: Range
persistent identity: range2484445
display id: range2484445
version: 1
top level: BBa_K1913001
direction: direction/forward
start: 1
end: 3
orientation: inline

chiB coding region
type: SequenceAnnotation
persistent identity: annotation2484448
display id: annotation2484448
version: 1
top level: BBa_K1913001
location: https://synbiohub.org/public/igem/BBa_K1913001/annotation2484448/range2484448/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1913001/annotation2484448/range2484448/1
type: Range
persistent identity: range2484448
display id: range2484448
version: 1
top level: BBa_K1913001
direction: direction/forward
start: 1
end: 1503
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2484446
display id: annotation2484446
version: 1
top level: BBa_K1913001
location: https://synbiohub.org/public/igem/BBa_K1913001/annotation2484446/range2484446/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1913001/annotation2484446/range2484446/1
type: Range
persistent identity: range2484446
display id: range2484446
version: 1
top level: BBa_K1913001
direction: direction/forward
start: 1498
end: 1500
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2484447
display id: annotation2484447
version: 1
top level: BBa_K1913001
location: https://synbiohub.org/public/igem/BBa_K1913001/annotation2484447/range2484447/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1913001/annotation2484447/range2484447/1
type: Range
persistent identity: range2484447
display id: range2484447
version: 1
top level: BBa_K1913001
direction: direction/forward
start: 1501
end: 1503
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1913003_sequence/1
type: Sequence
persistent identity: BBa K1913003 sequence
display id: BBa_K1913003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1913001_sequence/1
type: Sequence
persistent identity: BBa K1913001 sequence
display id: BBa_K1913001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccacacgtaaagccgttattgggtattattttattccaaccaaccaaatcaataattacaccgagtccgatacctccatcgtgccattcccggtatccaacatcacgccggccaaagccaaacagctgacgcacatcaacttctctttcctggatatcaacagcaacctggagtgcgcctgggatccggccaccaacgacgccaaagcgcgcgatgtggtcaagcgtctgaccgcgctcaaagcgcacaaccccaacctgcgcatcatgttctccatcggcggctggtactactccaacgatctgggcgtgtcgcacgccaactacgtcaacgcggtgaaaaccccggcgtcgcgcaccaagttcgcccaatcctgcgtacgcatcatgaaggattacggcttcgacggcgtggacatcgactgggagtatccgcaagcggcagaagtggacggcttcatcgccgcgctgcaagagatccgcaccctgctgaatcagcaaaccgttgctgacggccgccaagcactgccgtatcagttgaccatcgccggcgccggcggcgctttcttcctgtcgcgctattacagcaagctggcgcagatcgtcgcgccgctcgattacatcaacctgatgacctacgatctggccggcccctgggagaagatcaccaaccaccaggcggcgctgttcggcgacgcggccgggccgaccttctacaatgcgctgcgcgaagccaatctgggctggagctgggaagagctgacccgcgccttccccagcccgttcagcctgacggtcgatgccgcggtgcagcaacacctgatgatggaaggggtgccgagcgccaaaatcgtgatgggcgtgccgttttacggccgcgccttcaagggcgtcagcggcggcaacggcgggcaatacagcagccacagcacgccgggcgaagatccgtatccgagcaccgactactggctggtgggctgcgaagagtgtgtgcgcgacaaggatccgcgcatcgcgtcctatcgccagctggagcagatgcttcagggcaactacggctatcagcggttgtggaacgacaagaccaaaaccccttacctgtatcacgcgcagaacgggctgttcgtcacctatgacgacgccgagagcttcaaatacaaagcgaagtacatcaagcagcagcagctgggcggcgtgatgttctggcacctggggcaagacaaccgcaacggcgatctgctggccgcgctggatcgctatttcaacgccgcggactacgacgacagccagctggatatgggcaccggcctgcgctacaccggcgtcggccccggcaacctgccgatcatgacggcaccggcctacgtgccgggcaccatttacgcgcagggcgcgctggtgtcctatcagggttacgtctggcagaccaagtgggggtacatcacctctgcgccgggttcagacagcgcctggctgaaggtgggccgcgtagcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z