##gff-version 3
##sequence-region BBa_K1913005 1 2815
BBa_K1913005	.	engineered_region	852	2815	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_K1913005	.	engineered_region	2018	2815	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1913005	.	terminator	2687	2815	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1913005	.	terminator	2775	2815	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1913005	.	stem_loop	2782	2801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913005	.	polyA_site	2802	2815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913005	.	stop_codon	2808	2808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913005	.	engineered_region	2775	2815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1913005	.	terminator	2687	2766	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1913005	.	stem_loop	2698	2741	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913005	.	engineered_region	2687	2766	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913005	.	engineered_region	2018	2678	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1913005	.	CDS	2036	2653	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1913005	.	engineered_region	2036	2653	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1913005	.	engineered_tag	2654	2678	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1913005	.	CDS	2036	2614	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1913005	.	tag	2615	2646	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1913005	.	ribosome_entry_site	2018	2029	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1913005	.	conserved_region	2022	2025	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1913005	.	engineered_region	852	2009	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1913005	.	CDS	1029	1784	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1913005	.	engineered_region	1029	1030	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1913005	.	engineered_tag	1785	1809	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1913005	.	CDS	1029	1778	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1913005	.	point_mutation	1520	1520	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1913005	.	point_mutation	1202	1202	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1913005	.	engineered_region	1029	1784	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1913005	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1913005	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913005	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913005	.	ribosome_entry_site	1011	1022	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1913005	.	conserved_region	1015	1018	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1913005	.	promoter	1955	2009	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1913005	.	promoter	1974	1979	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1913005	.	engineered_region	1955	2009	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1913005	.	non_covalent_binding_site	1955	1974	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1913005	.	start_codon	2007	2007	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1913005	.	promoter	1996	2001	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1913005	.	promoter	852	1002	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1913005	.	engineered_region	852	1002	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1913005	.	start_codon	979	979	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1913005	.	promoter	966	973	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1913005	.	non_covalent_binding_site	981	1000	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1913005	.	promoter	940	945	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1913005	.	non_covalent_binding_site	852	871	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1913005	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1913005	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913005	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913005	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913005	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1913005	.	engineered_region	1	843	.	+	0	ID=BBa_K1913014;Name=BBa_K1913014
BBa_K1913005	.	engineered_region	56	843	.	+	0	ID=annotation2485139;Name=BBa_K081012;Parent=BBa_K1913014
BBa_K1913005	.	engineered_region	1	55	.	+	0	ID=annotation2485136;Name=BBa_R0062;Parent=BBa_K1913014
BBa_K1913005	.	ribosome_entry_site	56	70	.	+	0	ID=annotation2485138;Name=RBS;Parent=BBa_K1913014
BBa_K1913005	.	promoter	1	55	.	+	0	ID=annotation2485137;Name=lux pR;Parent=BBa_K1913014
BBa_K1913005	.	CDS	77	796	.	+	0	ID=annotation2497364;Name=GFP;Parent=BBa_K1913014
BBa_K1913005	.	sequence_feature	805	843	.	+	0	ID=annotation2497367;Name=misc;Parent=BBa_K1913014
>BBa_K1913005
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaatactagatg
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatttt
ctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacc
tgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacag
catgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactaca
agacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaga
tggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaat
ggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaata
ctccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaa
cgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
taataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagacctgtacgatcctacag
gtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatc
cattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaa
aatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagact
tgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgt
gatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctg
aattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctc
aaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatt
acagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggag
acaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagt
cttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata