##gff-version 3
##sequence-region BBa_K1913010 1 1773
BBa_K1913010	.	engineered_region	851	1773	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K1913010	.	terminator	1645	1724	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1913010	.	stem_loop	1656	1699	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913010	.	engineered_region	1645	1724	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913010	.	CDS	931	1611	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1913010	.	CDS	931	1605	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1913010	.	engineered_tag	1612	1636	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1913010	.	terminator	1733	1773	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1913010	.	stem_loop	1740	1759	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913010	.	polyA_site	1760	1773	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913010	.	stop_codon	1766	1766	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913010	.	engineered_region	1733	1773	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1913010	.	promoter	851	904	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1913010	.	engineered_region	851	904	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1913010	.	promoter	870	875	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1913010	.	non_covalent_binding_site	876	894	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1913010	.	promoter	893	898	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1913010	.	non_covalent_binding_site	851	869	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1913010	.	ribosome_entry_site	913	924	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1913010	.	conserved_region	917	920	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1913010	.	engineered_region	1	842	.	+	0	ID=BBa_P0140;Name=BBa_P0140
BBa_K1913010	.	CDS	21	680	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0140
BBa_K1913010	.	engineered_tag	681	705	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1913010	.	CDS	24	640	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1913010	.	tag	641	674	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1913010	.	terminator	714	793	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0140
BBa_K1913010	.	stem_loop	725	768	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913010	.	engineered_region	714	793	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913010	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0140
BBa_K1913010	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1913010	.	terminator	802	842	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0140
BBa_K1913010	.	stem_loop	809	828	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913010	.	polyA_site	829	842	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913010	.	stop_codon	835	835	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913010	.	engineered_region	802	842	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1913010
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagaga
ttgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata