LOCUS       BBa_K1913015            3285 bp    DNA     linear       26-Nov-2024
DEFINITION  Cry3Aa with araC/pBAD
ACCESSION   BBa_K1913015
VERSION     BBa_K1913015.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1234..1258
                     /label=rbs
     misc_feature    3260..3265
                     /label=stop
     misc_feature    3236..3259
                     /label=8xHis
     misc_feature    3215..3235
                     /label=TEV
     CDS             1219..3285
                     /label=BBa_K1913013
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagga attcgcggcc gcttctagag aaagatagga gacactagat
     1261 gataagaaag ggaggaagaa aaatgaatcc gaacaatcga agtgaacatg atacaataaa
     1321 aactactgaa aataatgagg tgccaactaa ccatgttcaa tatcctttag cggaaactcc
     1381 aaatccaaca ctagaagatt taaattataa agagttttta agaatgactg ctgataataa
     1441 tacggaagca ctagatagct ctacaacaaa agatgtcatt caaaaaggca tttccgtagt
     1501 aggtgatctc ctaggcgtag taggtttccc gtttggtgga gcgcttgttt cgttttatac
     1561 aaacttttta aatactattt ggccaagtga agacccgtgg aaggctttta tggaacaagt
     1621 agaagcattg atggatcaga aaatagctga ttatgcaaaa aataaagctc ttgcagagtt
     1681 acagggcctt caaaataatg tcgaagatta tgtgagtgca ttgagttcat ggcaaaaaaa
     1741 tcctgtgagt tcacgaaatc cacatagcca ggggcggata agagagctgt tttctcaagc
     1801 agaaagtcat tttcgtaatt caatgccttc gtttgcaatt tctggatacg aggttctatt
     1861 tctaacaaca tatgcacaag ctgccaacac acatttattt ttactaaaag acgctcaaat
     1921 ttatggagaa gaatggggat acgaaaaaga agatattgct gaattttata aaagacaact
     1981 aaaacttacg caagaatata ctgaccattg tgtcaaatgg tataatgttg gattagataa
     2041 attaagaggt tcatcttatg aatcttgggt aaactttaac cgttatcgca gagagatgac
     2101 attaacagta ttagatttaa ttgcactatt tccattgtat gatgttcggc tatacccaaa
     2161 agaagttaaa accgaattaa caagagacgt tttaacagat ccaattgtcg gagtcaacaa
     2221 ccttaggggc tatggaacaa ccttctctaa tatagaaaat tatattcgaa aaccacatct
     2281 atttgactat ctgcatagaa tccaatttca cacgcggttc caaccaggat attatggaaa
     2341 tgactctttc aattattggt ccggtaatta tgtttcaact agaccaagca taggatcaaa
     2401 tgatataatc acatctccat tctatggaaa taaatccagt gaacctgtac aaaatttaga
     2461 atttaatgga gaaaaagtct atagagccgt agcaaataca aatcttgcgg tctggccgtc
     2521 cgctgtatat tcaggtgtta caaaagtgga atttagccaa tataatgatc aaacagatga
     2581 agcaagtaca caaacgtacg actcaaaaag aaatgttggc gcggtcagct gggattctat
     2641 cgatcaattg cctccagaaa caacagatga acctctggaa aagggatata gccatcaact
     2701 caattatgta atgtgctttt taatgcaggg tagtagagga acaatcccag tgttaacttg
     2761 gacacataaa agtgtagact tttttaacat gattgattcg aaaaaaatta cacaacttcc
     2821 gttagtaaag gcatataagt tacaatctgg tgcttccgtt gtcgcaggtc ctaggtttac
     2881 aggaggagat atcattcaat gcacagaaaa tggaagtgcg gcaactattt acgttacacc
     2941 ggatgtgtcg tactctcaaa aatatcgagc tagaatccat tatgcttcta catctcagat
     3001 aacatttaca ctcagtttag acggggcacc atttaatcaa tactatttcg ataaaacgat
     3061 aaataaagga gacacattaa cgtataattc atttaattta gcaagtttca gcacaccatt
     3121 cgaattatca gggaataact tacaaatagg cgtcacagga ttaagtgctg gagataaagt
     3181 ttatatagac aaaattgaat ttattccagt gaatgagaac ttgtacttcc aggggcatca
     3241 ccatcaccac catcatcact aataaactag tagcggccgc tgcag
//