LOCUS       BBa_K1913027            1006 bp    DNA     linear       11-Dec-2025
DEFINITION  Wild type plac-FixK2 hybrid promoter with mRFP
ACCESSION   BBa_K1913027
VERSION     BBa_K1913027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Lac operator
     misc_feature    64..67
                     /label=FixJ box
     misc_feature    90..97
                     /label=FixJ box
     misc_feature    132..152
                     /label=Lac operator
     misc_feature    120..120
                     /label=-10
     misc_feature    98..98
                     /label=-35
     RBS             153..157
                     /label=RBS
     promoter        1..157
                     /label=BBa_K1913025
     CDS             164..838
                     /label=mrfp1
     misc_feature    845..869
                     /label=Help:Barcodes
     CDS             164..869
                     /label=BBa_E1010
     stem_loop       889..932
                     /label=stem_loop
     misc_feature    878..957
                     /label=BBa_B0010
     terminator      878..957
                     /label=BBa_B0010
     stem_loop       973..992
                     /label=T7 TE
     misc_feature    999..999
                     /label=stop
     polyA_site      993..1006
                     /label=polya
     misc_feature    966..1006
                     /label=BBa_B0012
     terminator      966..1006
                     /label=BBa_B0012
     terminator      878..1006
                     /label=BBa_B0015
ORIGIN
        1 ggcagtgagc gcaacgcaat tccatccagg accggcctcg ggcatgacct acggggttct
       61 acgtaaggca ccccccttaa gatatcgctc gaaattttcg aacctcccga taccgcgtac
      121 caatgcgtca taattgtgag cggataacaa ttggagatac tagatggctt cctccgaaga
      181 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
      241 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
      301 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
      361 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
      421 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
      481 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
      541 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
      601 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
      661 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
      721 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
      781 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
      841 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
      901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      961 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//