LOCUS       BBa_K1913029            1005 bp    DNA     linear       16-Jun-2024
DEFINITION  Synthetic plac-FixK2 hybrid promoter +RBS with mRFP
ACCESSION   BBa_K1913029
VERSION     BBa_K1913029.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     misc_feature    145..156
                     /label=BBa_B0034
     misc_feature    41..48
                     /label=FixJ box
     misc_feature    75..88
                     /label=FixJ box
     misc_feature    89..123
                     /label=BBa_J23106
     misc_feature    124..144
                     /label=lac operator
     misc_feature    1..21
                     /label=lac operator
     promoter        1..156
                     /label=BBa_K1913022
     CDS             163..837
                     /label=mrfp1
     misc_feature    844..868
                     /label=Help:Barcodes
     CDS             163..868
                     /label=BBa_E1010
     stem_loop       888..931
                     /label=stem_loop
     misc_feature    877..956
                     /label=BBa_B0010
     terminator      877..956
                     /label=BBa_B0010
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     terminator      877..1005
                     /label=BBa_B0015
ORIGIN
        1 ggcagtgagc gcaacgcaat tccatccagg accggcctcg gtacgtagcc ggcctcgggc
       61 atgacctacg gggtttgatc tgggtcaatt tacggctagc tcagtcctag gtatagtgct
      121 agcaattgtg agcggataac aattaaagag gagaaatact agatggcttc ctccgaagac
      181 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
      241 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
      301 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
      361 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
      421 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
      481 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
      541 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
      601 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
      661 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
      721 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
      781 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
      841 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//