LOCUS       BBa_K1913030            1011 bp    DNA     linear       26-Nov-2024
DEFINITION  Synthetic plac-FixK2 hybrid promoter+RBS with mRFP
ACCESSION   BBa_K1913030
VERSION     BBa_K1913030.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     misc_feature    151..162
                     /label=BBa_B0034
     misc_feature    75..88
                     /label=FixJ box
     misc_feature    41..48
                     /label=FixJ box
     misc_feature    1..21
                     /label=lac operator
     misc_feature    89..129
                     /label=ompC core element
     misc_feature    130..150
                     /label=lac operator
     promoter        1..162
                     /label=BBa_K1913023
     CDS             169..843
                     /label=mrfp1
     misc_feature    850..874
                     /label=Help:Barcodes
     CDS             169..874
                     /label=BBa_E1010
     stem_loop       894..937
                     /label=stem_loop
     misc_feature    883..962
                     /label=BBa_B0010
     terminator      883..962
                     /label=BBa_B0010
     stem_loop       978..997
                     /label=T7 TE
     misc_feature    1004..1004
                     /label=stop
     polyA_site      998..1011
                     /label=polya
     misc_feature    971..1011
                     /label=BBa_B0012
     terminator      971..1011
                     /label=BBa_B0012
     terminator      883..1011
                     /label=BBa_B0015
ORIGIN
        1 ggcagtgagc gcaacgcaat tccatccagg accggcctcg gtacgtagcc ggcctcgggc
       61 atgacctacg gggtttgatc tgggtcaaat tcgtgttgga ttattctgca tttttgggga
      121 gaatggacta attgtgagcg gataacaatt aaagaggaga aatactagat ggcttcctcc
      181 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      241 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      301 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      361 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      421 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      481 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      541 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      601 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
      661 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
      721 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
      781 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      841 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac
      901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      961 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//