LOCUS       BBa_K1913031             984 bp    DNA     linear       16-Jun-2024
DEFINITION  Synthetic ptet-FixK2 hybrid promoter+RBS with mRFP
ACCESSION   BBa_K1913031
VERSION     BBa_K1913031.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     misc_feature    124..135
                     /label=BBa_B0034
     misc_feature    20..27
                     /label=FixJ box
     misc_feature    54..67
                     /label=FixJ box
     misc_feature    74..92
                     /label=tet operator
     misc_feature    99..117
                     /label=	tet operator
     misc_feature    93..98
                     /label=-10
     misc_feature    68..73
                     /label=-35
     promoter        1..135
                     /label=BBa_K1913024
     CDS             142..816
                     /label=mrfp1
     misc_feature    823..847
                     /label=Help:Barcodes
     CDS             142..847
                     /label=BBa_E1010
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     terminator      856..984
                     /label=BBa_B0015
ORIGIN
        1 ccatccagga ccggcctcgg tacgtagccg gcctcgggca tgacctacgg ggtttgatct
       61 gggtcaattt acgtccctat cagtgataga gatatagttc cctatcagtg atagagagct
      121 agcaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      181 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      241 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      301 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      361 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      421 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      481 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      541 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      601 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      661 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      721 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      781 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tata
//