LOCUS BBa_K1913032 3468 bp DNA linear 17-Dec-2024 DEFINITION Toggle Switch device ACCESSION BBa_K1913032 VERSION BBa_K1913032.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 stem_loop 209..289 /label=G box misc_feature 288..294 /label=Anti-terminator misc_feature 296..302 /label=Anti-terminator stem_loop 337..397 /label=terminator misc_feature 345..352 /label=Anti-terminator misc_feature 354..360 /label=Anti-terminator misc_feature 209..397 /label=BBa_K1913019 misc_feature 410..413 /label=conserved RBS 406..417 /label=BBa_B0034 CDS 427..1043 /label=tetR misc_feature 1044..1077 /label=SsrA misc_feature 1084..1108 /label=Help:Barcodes CDS 424..1083 /label=BBa_C0040 stem_loop 1128..1171 /label=stem_loop misc_feature 1117..1196 /label=BBa_B0010 terminator 1117..1196 /label=BBa_B0010 stem_loop 1212..1231 /label=T7 TE misc_feature 1238..1238 /label=stop polyA_site 1232..1245 /label=polya misc_feature 1205..1245 /label=BBa_B0012 terminator 1205..1245 /label=BBa_B0012 misc_feature 406..1245 /label=BBa_P0440 misc_feature 1381..1384 /label=conserved RBS 1377..1388 /label=BBa_B0034 misc_feature 1258..1261 /label=conserved misc_feature 1377..1388 /label=BBa_B0034 misc_feature 1273..1280 /label=FixJ box misc_feature 1307..1320 /label=FixJ box misc_feature 1327..1345 /label=tet operator misc_feature 1352..1370 /label= tet operator misc_feature 1346..1351 /label=-10 misc_feature 1321..1326 /label=-35 promoter 1254..1388 /label=BBa_K1913024 RBS 1397..1408 /label=B0034 CDS 1415..2089 /label=E1010 misc_feature 2090..2107 /label=6xHis tag misc_feature 2113..2152 /label=M0050 misc_feature 1397..2152 /label=BBa_K1913020 misc_feature 2165..2168 /label=conserved RBS 2161..2172 /label=BBa_B0034 misc_feature 3268..3306 /label=LVA CDS 2179..3306 /label=lacI-LVA misc_feature 2179..3306 /label=BBa_C0012 misc_feature 3307..3331 /label=Help:Barcodes CDS 2179..3306 /label=BBa_C0012 stem_loop 3351..3394 /label=stem_loop misc_feature 3340..3419 /label=BBa_B0010 terminator 3340..3419 /label=BBa_B0010 stem_loop 3435..3454 /label=T7 TE misc_feature 3461..3461 /label=stop polyA_site 3455..3468 /label=polya misc_feature 3428..3468 /label=BBa_B0012 terminator 3428..3468 /label=BBa_B0012 misc_feature 2161..3468 /label=BBa_P0412 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagag gaaaaagaca ttcttgtata tgatcagtaa 241 tatggtctga ttgtttctac ctagtaaccg taaaaaacta gactacaaga aagtttgaaa 301 atttgaacga gttgaaaagg acaagttctt ttctgtttgc tcttattttt cacactttct 361 gcacttccag aatttgtgaa ggataagagc ttttttttac tagagaaaga ggagaaatac 421 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 481 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 541 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 601 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 661 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 721 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 781 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 841 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 901 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 961 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 1021 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 1081 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1201 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagccatcca 1261 ggaccggcct cggtacgtag ccggcctcgg gcatgaccta cggggtttga tctgggtcaa 1321 tttacgtccc tatcagtgat agagatatag ttccctatca gtgatagaga gctagcaaag 1381 aggagaaata ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa 1441 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat 1501 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac 1561 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc 1621 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga 1681 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca 1741 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt 1801 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg 1861 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga 1921 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct 1981 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac 2041 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctc atcatcacca 2101 ccatcactaa taagctgcta acgacgaaaa ctacgctctg gctgcttaat aatactagag 2161 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 2221 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 2281 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 2341 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 2401 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 2461 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 2521 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 2581 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 2641 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 2701 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 2761 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 2821 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 2881 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 2941 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 3001 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 3061 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 3121 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 3181 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 3241 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 3301 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 3361 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3421 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //