BBa_K1913032
type: ComponentDefinition
persistent identity: BBa K1913032
display id: BBa_K1913032
version: 1
was generated by: iGEM to SBOL conversion
description: Toggle Switch device
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T07:42:11Z
mutable provenance: Genomic sequence of Bradyrhizobium japonicum, iGEM part registry
owned by: james
owned by: myers
mutable description: The toggle switch controls expression of the BeeT???s toxin between an off-state and an on-state, which can be switched with two stimulus, guanine (or vitamin B12) and blue light.
discontinued: false
dominant: false
group u list: _2380_
m user id: 29979
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: we constructed a regulatory system that could be applied into real beehive and it integrates mite sensing with light-control device, which could provide a stable output of toxin expression with fast responsiveness.
star: false
creator: Tianhe Wang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_P0412
component: BBa_K1913020
component: BBa_R0010
component: BBa_K1913024
component: BBa_B0034
component: BBa_P0440
component: BBa_K1913019
sequence annotation: BBa_R0010
sequence annotation: BBa_K1913019
sequence annotation: BBa_P0440
sequence annotation: BBa_K1913024
sequence annotation: BBa_B0034
sequence annotation: BBa_K1913020
sequence annotation: BBa_P0412
sequence: https://synbiohub.org/public/igem/BBa_K1913032_sequence/1

BBa_P0412
type: Component
persistent identity: component2505225
display id: component2505225
version: 1
top level: BBa_K1913032
definition: BBa_P0412
access: public

BBa_K1913020
type: Component
persistent identity: component2505212
display id: component2505212
version: 1
top level: BBa_K1913032
definition: BBa_K1913020
access: public

BBa_R0010
type: Component
persistent identity: component2505178
display id: component2505178
version: 1
top level: BBa_K1913032
definition: LacI
access: public

BBa_K1913024
type: Component
persistent identity: component2505207
display id: component2505207
version: 1
top level: BBa_K1913032
definition: BBa_K1913024
access: public

BBa_B0034
type: Component
persistent identity: component2505206
display id: component2505206
version: 1
top level: BBa_K1913032
definition: BBa_B0034
access: public

BBa_P0440
type: Component
persistent identity: component2505198
display id: component2505198
version: 1
top level: BBa_K1913032
definition: BBa_P0440
access: public

BBa_K1913019
type: Component
persistent identity: component2505185
display id: component2505185
version: 1
top level: BBa_K1913032
definition: BBa_K1913019
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2505178
display id: annotation2505178
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505178/range2505178/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505178/range2505178/1
type: Range
persistent identity: range2505178
display id: range2505178
version: 1
top level: BBa_K1913032
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1913019
type: SequenceAnnotation
persistent identity: annotation2505185
display id: annotation2505185
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505185/range2505185/1
component: BBa_K1913019

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505185/range2505185/1
type: Range
persistent identity: range2505185
display id: range2505185
version: 1
top level: BBa_K1913032
direction: direction/reverse
start: 209
end: 397
orientation: inline

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2505198
display id: annotation2505198
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505198/range2505198/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505198/range2505198/1
type: Range
persistent identity: range2505198
display id: range2505198
version: 1
top level: BBa_K1913032
direction: direction/reverse
start: 406
end: 1245
orientation: inline

BBa_K1913024
type: SequenceAnnotation
persistent identity: annotation2505207
display id: annotation2505207
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505207/range2505207/1
component: BBa_K1913024

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505207/range2505207/1
type: Range
persistent identity: range2505207
display id: range2505207
version: 1
top level: BBa_K1913032
direction: direction/reverse
start: 1254
end: 1388
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2505206
display id: annotation2505206
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505206/range2505206/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505206/range2505206/1
type: Range
persistent identity: range2505206
display id: range2505206
version: 1
top level: BBa_K1913032
direction: direction/forward
start: 1377
end: 1388
orientation: inline

BBa_K1913020
type: SequenceAnnotation
persistent identity: annotation2505212
display id: annotation2505212
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505212/range2505212/1
component: BBa_K1913020

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505212/range2505212/1
type: Range
persistent identity: range2505212
display id: range2505212
version: 1
top level: BBa_K1913032
direction: direction/reverse
start: 1397
end: 2152
orientation: inline

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2505225
display id: annotation2505225
version: 1
top level: BBa_K1913032
location: https://synbiohub.org/public/igem/BBa_K1913032/annotation2505225/range2505225/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K1913032/annotation2505225/range2505225/1
type: Range
persistent identity: range2505225
display id: range2505225
version: 1
top level: BBa_K1913032
direction: direction/reverse
start: 2161
end: 3468
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_P0412
type: ComponentDefinition
persistent identity: BBa P0412
display id: BBa_P0412
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> LacI [S0150]
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_C0012
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0412_sequence/1

BBa_C0012
type: Component
persistent identity: component944837
display id: component944837
version: 1
top level: BBa_P0412
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component944827
display id: component944827
version: 1
top level: BBa_P0412
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component944854
display id: component944854
version: 1
top level: BBa_P0412
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944844
display id: component944844
version: 1
top level: BBa_P0412
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944827
display id: annotation944827
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
type: Range
persistent identity: range944827
display id: range944827
version: 1
top level: BBa_P0412
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944837
display id: annotation944837
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
type: Range
persistent identity: range944837
display id: range944837
version: 1
top level: BBa_P0412
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944844
display id: annotation944844
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
type: Range
persistent identity: range944844
display id: range944844
version: 1
top level: BBa_P0412
direction: direction/forward
start: 1180
end: 1259
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944854
display id: annotation944854
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
type: Range
persistent identity: range944854
display id: range944854
version: 1
top level: BBa_P0412
direction: direction/forward
start: 1268
end: 1308
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> TetR [S0151]
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
top level: BBa_P0440
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
top level: BBa_P0440
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
top level: BBa_P0440
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
top level: BBa_P0440
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_K1913020
type: ComponentDefinition
persistent identity: BBa K1913020
display id: BBa_K1913020
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP with degradation tag
created: 2016-10-06T11:00:00Z
modified: 2016-10-07T07:20:00Z
mutable provenance: iGEM part registry
owned by: james
owned by: myers
mutable description: mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.
discontinued: false
dominant: false
group u list: _2380_
m user id: 29979
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Making a mRFP coding sequence with fast degradation tag, which is easy for people to use it.
star: false
creator: Tianhe Wang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: B0034
sequence annotation: E1010
sequence annotation: 6xHis tag
sequence annotation: M0050
sequence: https://synbiohub.org/public/igem/BBa_K1913020_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2487316
display id: annotation2487316
version: 1
top level: BBa_K1913020
location: https://synbiohub.org/public/igem/BBa_K1913020/annotation2487316/range2487316/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1913020/annotation2487316/range2487316/1
type: Range
persistent identity: range2487316
display id: range2487316
version: 1
top level: BBa_K1913020
direction: direction/forward
start: 1
end: 12
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2487317
display id: annotation2487317
version: 1
top level: BBa_K1913020
location: https://synbiohub.org/public/igem/BBa_K1913020/annotation2487317/range2487317/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1913020/annotation2487317/range2487317/1
type: Range
persistent identity: range2487317
display id: range2487317
version: 1
top level: BBa_K1913020
direction: direction/forward
start: 19
end: 693
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2487318
display id: annotation2487318
version: 1
top level: BBa_K1913020
location: https://synbiohub.org/public/igem/BBa_K1913020/annotation2487318/range2487318/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1913020/annotation2487318/range2487318/1
type: Range
persistent identity: range2487318
display id: range2487318
version: 1
top level: BBa_K1913020
direction: direction/forward
start: 694
end: 711
orientation: inline

M0050
type: SequenceAnnotation
persistent identity: annotation2487319
display id: annotation2487319
version: 1
top level: BBa_K1913020
location: https://synbiohub.org/public/igem/BBa_K1913020/annotation2487319/range2487319/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1913020/annotation2487319/range2487319/1
type: Range
persistent identity: range2487319
display id: range2487319
version: 1
top level: BBa_K1913020
direction: direction/forward
start: 717
end: 756
orientation: inline

BBa_K1913024
type: ComponentDefinition
persistent identity: BBa K1913024
display id: BBa_K1913024
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ptet-FixK2 hybrid promoter with RBS
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T03:46:38Z
mutable provenance: genomic sequence of Bradyrhizobium japonicum
owned by: james
owned by: myers
mutable description: Synthetic ptet-FixK2 hybrid promoter is the promoter that could not only be activated by light sensors YF1-FixJ composite and but inhibited by TetR repressor. The sequence of upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. And core element region is replaced by core element region of BBa_J23106. Addition tet operators are inserted into core element region of promoter as second control element that results in transcription repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _2380_
m user id: 29556
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: According to some previous iGEM projects (UNITN-Trento 2013, INSA-Toulouse 2013), the transcription activity of the wild type Fixk2 promoter is so weak that they all added an inverter part to control their target gene expression. Even the original pDusk system in darkness has only 5 times expression levels than in light conditions. Therefore, we decided to enhance the transcriptional activity of the Fixk2 promoter by changing the core element region of the wild type Fixk2 by adding a strong constitutive promoter (BBa_J23106) from iGEM part registry and by adding two typical FixJ boxes into the -40 to -70 region.
star: false
creator: Tianhe Wang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: conserved
sequence annotation: FixJ box
sequence annotation: -35
sequence annotation: tet operator
sequence annotation: -10
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1913024_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2502758
display id: annotation2502758
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502758/range2502758/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502758/range2502758/1
type: Range
persistent identity: range2502758
display id: range2502758
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 5
end: 8
orientation: inline

FixJ box
type: SequenceAnnotation
persistent identity: annotation2502761
display id: annotation2502761
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502761/range2502761/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502761/range2502761/1
type: Range
persistent identity: range2502761
display id: range2502761
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 20
end: 27
orientation: inline

FixJ box
type: SequenceAnnotation
persistent identity: annotation2502762
display id: annotation2502762
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502762/range2502762/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502762/range2502762/1
type: Range
persistent identity: range2502762
display id: range2502762
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 54
end: 67
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2502785
display id: annotation2502785
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502785/range2502785/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502785/range2502785/1
type: Range
persistent identity: range2502785
display id: range2502785
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 68
end: 73
orientation: inline

tet operator
type: SequenceAnnotation
persistent identity: annotation2502777
display id: annotation2502777
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502777/range2502777/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502777/range2502777/1
type: Range
persistent identity: range2502777
display id: range2502777
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 74
end: 92
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2502783
display id: annotation2502783
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502783/range2502783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502783/range2502783/1
type: Range
persistent identity: range2502783
display id: range2502783
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 93
end: 98
orientation: inline

tet operator
type: SequenceAnnotation
persistent identity: annotation2502781
display id: annotation2502781
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502781/range2502781/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502781/range2502781/1
type: Range
persistent identity: range2502781
display id: range2502781
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 99
end: 117
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2502759
display id: annotation2502759
version: 1
top level: BBa_K1913024
location: https://synbiohub.org/public/igem/BBa_K1913024/annotation2502759/range2502759/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1913024/annotation2502759/range2502759/1
type: Range
persistent identity: range2502759
display id: range2502759
version: 1
top level: BBa_K1913024
direction: direction/forward
start: 124
end: 135
orientation: inline

BBa_K1913019
type: ComponentDefinition
persistent identity: BBa K1913019
display id: BBa_K1913019
version: 1
was generated by: iGEM to SBOL conversion
description: Guanine riboswitch BS-yxjA
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T07:37:54Z
mutable provenance: Genomic sequence of B. subtilis
owned by: james
owned by: myers
mutable description: Guanine riboswitches BS-yxjA negatively modulate transcription upon guanine binding.BS-yxjA variant displays a higher 2AP affinity with an increase of 13-fold relative to other variants in vitro test.
discontinued: false
dominant: false
group u list: _2380_
m user id: 29979
owner id: 29979
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: According to Mulhbacher J et al, riboswitches BS-yxjA showed wide range sensitivity of Guanine concentration in vitro test.
star: false
creator: Tianhe Wang
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: G box
sequence annotation: Anti-terminator
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K1913019_sequence/1

G box
type: SequenceAnnotation
persistent identity: annotation2505991
display id: annotation2505991
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505991/range2505991/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505991/range2505991/1
type: Range
persistent identity: range2505991
display id: range2505991
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 1
end: 81
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2505992
display id: annotation2505992
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505992/range2505992/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505992/range2505992/1
type: Range
persistent identity: range2505992
display id: range2505992
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 80
end: 86
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2505993
display id: annotation2505993
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505993/range2505993/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505993/range2505993/1
type: Range
persistent identity: range2505993
display id: range2505993
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 88
end: 94
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2505994
display id: annotation2505994
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2505994/range2505994/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1913019/annotation2505994/range2505994/1
type: Range
persistent identity: range2505994
display id: range2505994
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 129
end: 189
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2506007
display id: annotation2506007
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2506007/range2506007/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913019/annotation2506007/range2506007/1
type: Range
persistent identity: range2506007
display id: range2506007
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 137
end: 144
orientation: inline

Anti-terminator
type: SequenceAnnotation
persistent identity: annotation2506009
display id: annotation2506009
version: 1
top level: BBa_K1913019
location: https://synbiohub.org/public/igem/BBa_K1913019/annotation2506009/range2506009/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1913019/annotation2506009/range2506009/1
type: Range
persistent identity: range2506009
display id: range2506009
version: 1
top level: BBa_K1913019
direction: direction/forward
start: 146
end: 152
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_K1913032_sequence/1
type: Sequence
persistent identity: BBa K1913032 sequence
display id: BBa_K1913032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaggaaaaagacattcttgtatatgatcagtaatatggtctgattgtttctacctagtaaccgtaaaaaactagactacaagaaagtttgaaaatttgaacgagttgaaaaggacaagttcttttctgtttgctcttatttttcacactttctgcacttccagaatttgtgaaggataagagcttttttttactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccatccaggaccggcctcggtacgtagccggcctcgggcatgacctacggggtttgatctgggtcaatttacgtccctatcagtgatagagatatagttccctatcagtgatagagagctagcaaagaggagaaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0412_sequence/1
type: Sequence
persistent identity: BBa P0412 sequence
display id: BBa_P0412_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0440_sequence/1
type: Sequence
persistent identity: BBa P0440 sequence
display id: BBa_P0440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1913020_sequence/1
type: Sequence
persistent identity: BBa K1913020 sequence
display id: BBa_K1913020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataagctgctaacgacgaaaactacgctctggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1913024_sequence/1
type: Sequence
persistent identity: BBa K1913024 sequence
display id: BBa_K1913024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatccaggaccggcctcggtacgtagccggcctcgggcatgacctacggggtttgatctgggtcaatttacgtccctatcagtgatagagatatagttccctatcagtgatagagagctagcaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1913019_sequence/1
type: Sequence
persistent identity: BBa K1913019 sequence
display id: BBa_K1913019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaaaaagacattcttgtatatgatcagtaatatggtctgattgtttctacctagtaaccgtaaaaaactagactacaagaaagtttgaaaatttgaacgagttgaaaaggacaagttcttttctgtttgctcttatttttcacactttctgcacttccagaatttgtgaaggataagagcttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z