LOCUS BBa_K1913033 3470 bp DNA linear 26-Nov-2024 DEFINITION Toggle Switch device ACCESSION BBa_K1913033 VERSION BBa_K1913033.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 stem_loop 209..289 /label=G box misc_feature 288..294 /label=Anti-terminator misc_feature 296..302 /label=Anti-terminator stem_loop 337..397 /label=terminator misc_feature 345..352 /label=Anti-terminator misc_feature 354..360 /label=Anti-terminator misc_feature 209..397 /label=BBa_K1913019 misc_feature 410..413 /label=conserved RBS 406..417 /label=BBa_B0034 CDS 427..1043 /label=tetR misc_feature 1044..1077 /label=SsrA misc_feature 1084..1108 /label=Help:Barcodes CDS 424..1083 /label=BBa_C0040 stem_loop 1128..1171 /label=stem_loop misc_feature 1117..1196 /label=BBa_B0010 terminator 1117..1196 /label=BBa_B0010 stem_loop 1212..1231 /label=T7 TE misc_feature 1238..1238 /label=stop polyA_site 1232..1245 /label=polya misc_feature 1205..1245 /label=BBa_B0012 terminator 1205..1245 /label=BBa_B0012 misc_feature 406..1245 /label=BBa_P0440 misc_feature 1296..1299 /label=FixJ box misc_feature 1322..1329 /label=FixJ box misc_feature 1335..1353 /label=tet operator misc_feature 1360..1378 /label=tet operator RBS 1386..1390 /label=RBS promoter 1254..1390 /label=BBa_K1913026 RBS 1399..1410 /label=B0034 CDS 1417..2091 /label=E1010 misc_feature 2092..2109 /label=6xHis tag misc_feature 2115..2154 /label=M0050 misc_feature 1399..2154 /label=BBa_K1913020 misc_feature 2167..2170 /label=conserved RBS 2163..2174 /label=BBa_B0034 misc_feature 3270..3308 /label=LVA CDS 2181..3308 /label=lacI-LVA misc_feature 2181..3308 /label=BBa_C0012 misc_feature 3309..3333 /label=Help:Barcodes CDS 2181..3308 /label=BBa_C0012 stem_loop 3353..3396 /label=stem_loop misc_feature 3342..3421 /label=BBa_B0010 terminator 3342..3421 /label=BBa_B0010 stem_loop 3437..3456 /label=T7 TE misc_feature 3463..3463 /label=stop polyA_site 3457..3470 /label=polya misc_feature 3430..3470 /label=BBa_B0012 terminator 3430..3470 /label=BBa_B0012 misc_feature 2163..3470 /label=BBa_P0412 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagag gaaaaagaca ttcttgtata tgatcagtaa 241 tatggtctga ttgtttctac ctagtaaccg taaaaaacta gactacaaga aagtttgaaa 301 atttgaacga gttgaaaagg acaagttctt ttctgtttgc tcttattttt cacactttct 361 gcacttccag aatttgtgaa ggataagagc ttttttttac tagagaaaga ggagaaatac 421 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 481 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 541 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 601 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 661 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 721 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 781 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 841 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 901 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 961 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 1021 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 1081 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1201 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagccatcca 1261 ggaccggcct cgggcatgac ctacggggtt ctacgtaagg cacccccctt aagatatcgc 1321 tcgaaatttt cgaatcccta tcagtgatag agaaccaatt ccctatcagt gatagagagc 1381 gtctaggaga tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 1441 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 1501 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 1561 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 1621 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 1681 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 1741 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 1801 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 1861 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 1921 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 1981 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 2041 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc tcatcatcac 2101 caccatcact aataagctgc taacgacgaa aactacgctc tggctgctta ataatactag 2161 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 2221 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 2281 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 2341 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 2401 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 2461 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 2521 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 2581 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 2641 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 2701 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 2761 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 2821 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 2881 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 2941 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 3001 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 3061 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 3121 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 3181 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3241 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 3301 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 3361 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3421 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //