##gff-version 3
##sequence-region BBa_K1913033 1 3470
BBa_K1913033	.	engineered_region	2163	3470	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K1913033	.	CDS	2181	3308	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K1913033	.	engineered_region	2181	3308	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1913033	.	CDS	2181	3308	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1913033	.	engineered_tag	3309	3333	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1913033	.	tag	3270	3308	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1913033	.	terminator	3342	3421	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K1913033	.	stem_loop	3353	3396	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913033	.	engineered_region	3342	3421	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913033	.	terminator	3430	3470	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K1913033	.	polyA_site	3457	3470	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913033	.	stem_loop	3437	3456	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913033	.	engineered_region	3430	3470	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1913033	.	stop_codon	3463	3463	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913033	.	ribosome_entry_site	2163	2174	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K1913033	.	conserved_region	2167	2170	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1913033	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1913033	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1913033	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1913033	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1913033	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1913033	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1913033	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1913033	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1913033	.	promoter	1254	1390	.	+	0	ID=BBa_K1913026;Name=BBa_K1913026
BBa_K1913033	.	non_covalent_binding_site	1296	1299	.	+	0	ID=annotation2501545;Name=FixJ box;Parent=BBa_K1913026
BBa_K1913033	.	operator	1335	1353	.	+	0	ID=annotation2501548;Name=tet operator;Parent=BBa_K1913026
BBa_K1913033	.	non_covalent_binding_site	1322	1329	.	+	0	ID=annotation2501547;Name=FixJ box;Parent=BBa_K1913026
BBa_K1913033	.	ribosome_entry_site	1386	1390	.	+	0	ID=annotation2501564;Name=RBS;Parent=BBa_K1913026
BBa_K1913033	.	operator	1360	1378	.	+	0	ID=annotation2501563;Name=tet operator;Parent=BBa_K1913026
BBa_K1913033	.	engineered_region	1399	2154	.	+	0	ID=BBa_K1913020;Name=BBa_K1913020
BBa_K1913033	.	CDS	1417	2091	.	+	0	ID=annotation2487317;Name=E1010;Parent=BBa_K1913020
BBa_K1913033	.	tag	2115	2154	.	+	0	ID=annotation2487319;Name=M0050;Parent=BBa_K1913020
BBa_K1913033	.	ribosome_entry_site	1399	1410	.	+	0	ID=annotation2487316;Name=B0034;Parent=BBa_K1913020
BBa_K1913033	.	tag	2092	2109	.	+	0	ID=annotation2487318;Name=6xHis tag;Parent=BBa_K1913020
BBa_K1913033	.	engineered_region	406	1245	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1913033	.	terminator	1117	1196	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1913033	.	stem_loop	1128	1171	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1913033	.	engineered_region	1117	1196	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1913033	.	ribosome_entry_site	406	417	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1913033	.	conserved_region	410	413	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1913033	.	terminator	1205	1245	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1913033	.	polyA_site	1232	1245	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1913033	.	stem_loop	1212	1231	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1913033	.	engineered_region	1205	1245	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1913033	.	stop_codon	1238	1238	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1913033	.	CDS	424	1083	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1913033	.	CDS	427	1043	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1913033	.	engineered_tag	1084	1108	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1913033	.	tag	1044	1077	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1913033	.	mature_transcript_region	209	397	.	+	0	ID=BBa_K1913019;Name=BBa_K1913019
BBa_K1913033	.	stem_loop	337	397	.	+	0	ID=annotation2505994;Name=terminator;Parent=BBa_K1913019
BBa_K1913033	.	non_covalent_binding_site	296	302	.	+	0	ID=annotation2505993;Name=Anti-terminator;Parent=BBa_K1913019
BBa_K1913033	.	stem_loop	209	289	.	+	0	ID=annotation2505991;Name=G box;Parent=BBa_K1913019
BBa_K1913033	.	non_covalent_binding_site	288	294	.	+	0	ID=annotation2505992;Name=Anti-terminator;Parent=BBa_K1913019
BBa_K1913033	.	non_covalent_binding_site	354	360	.	+	0	ID=annotation2506009;Name=Anti-terminator;Parent=BBa_K1913019
BBa_K1913033	.	non_covalent_binding_site	345	352	.	+	0	ID=annotation2506007;Name=Anti-terminator;Parent=BBa_K1913019
>BBa_K1913033
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaggaaaaagacattcttgtatatgatcag
taatatggtctgattgtttctacctagtaaccgtaaaaaactagactacaagaaagtttgaaaatttgaacgagttgaa
aaggacaagttcttttctgtttgctcttatttttcacactttctgcacttccagaatttgtgaaggataagagcttttt
tttactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgctt
aatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggc
atgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgcccttt
agaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatgga
gcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaac
aaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatca
agagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaactta
aatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccatccaggac
cggcctcgggcatgacctacggggttctacgtaaggcaccccccttaagatatcgctcgaaattttcgaatccctatca
gtgatagagaaccaattccctatcagtgatagagagcgtctaggagatactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataagctgctaacgacgaaaac
tacgctctggctgcttaataatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacga
tgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacg
cgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagt
cgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgc
cgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaat
cttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcct
gcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacgg
tacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtc
tcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcg
actggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgc
caacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtg
ggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggc
aaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggt
gaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
cgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagt
gctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata